Department of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, VIC, 3086, Australia.
Department of Biochemistry and Genetics, La Trobe Institute for Molecular Sciences, La Trobe University, Bundoora, VIC, 3086, Australia.
Proteomics. 2019 Jan;19(1-2):e1800209. doi: 10.1002/pmic.201800209. Epub 2018 Dec 17.
Gram-negative bacteria release outer membrane vesicles (OMVs) as part of their normal growth that contain a range of cargo from their parent bacterium, including DNA, RNA, and proteins. The protein content of OMVs is suggested to be similar in composition to various sub-cellular locations of their parent bacterium. However, very little is known regarding the effect of bacterial growth stage on the size, content, and selective packaging of proteins into OMVs. In this study, the global proteome of Helicobacter pylori and their OMVs throughout bacterial growth are examined to determine if bacterial growth stage affected OMV cargo composition. Analysis of OMVs produced by H. pylori reveals that bacterial growth stage affects the size, composition, and selection of protein cargo into OMVs. Proteomic analysis identifies that the proteome of H. pylori OMVs is vastly different throughout bacterial growth and that OMVs contain a range of proteins compared to their parent bacteria. In addition, bacterial growth stage affects the ability of OMVs to induce the production of IL-8 by human epithelial cells. Therefore, the findings identify that the size, proteome, and immunogenicity of OMVs produced during various stages of bacterial growth is not comparable. Collectively, these findings highlight the importance of considering the bacterial growth stage from which OMVs are isolated, as this will impact their size, protein composition, and ultimately their biological functions.
革兰氏阴性菌在正常生长过程中会释放出含有来自母菌各种货物（包括 DNA、RNA 和蛋白质）的外膜囊泡（OMV）。OMV 中的蛋白质含量在组成上与母菌的各种亚细胞位置相似。然而，关于细菌生长阶段对 OMV 中蛋白质的大小、含量和选择性包装的影响，人们知之甚少。在这项研究中，研究了幽门螺杆菌的全蛋白质组及其在细菌生长过程中的 OMV，以确定细菌生长阶段是否影响 OMV 货物组成。对幽门螺杆菌产生的 OMV 的分析表明，细菌生长阶段影响 OMV 货物的大小、组成和选择。蛋白质组学分析表明，OMV 的蛋白质组在整个细菌生长过程中存在很大差异，并且 OMV 与母菌相比包含多种蛋白质。此外，细菌生长阶段影响 OMV 诱导人上皮细胞产生 IL-8 的能力。因此，这些发现表明，在细菌生长的不同阶段产生的 OMV 的大小、蛋白质组和免疫原性是不可比的。总之，这些发现强调了考虑从 OMV 分离的细菌生长阶段的重要性，因为这将影响它们的大小、蛋白质组成，最终影响它们的生物学功能。
