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从沙特阿拉伯利雅得和卡西姆的阿拉伯骆驼()中分离出的分子鉴定。

Molecular Identification of Isolated from Arabian Camels () in Riyadh and Al-Qassim, Saudi Arabia.

作者信息

Metwally Dina M, Al-Turaiki Isra M, Altwaijry Najwa, Alghamdi Samia Q, Alanazi Abdullah D

机构信息

Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.

Department of Parasitology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.

出版信息

Animals (Basel). 2021 Apr 17;11(4):1149. doi: 10.3390/ani11041149.

Abstract

We analyzed the blood from 400 one-humped camels, () in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite spp. Polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) gene was used to detect the prevalence of spp. in the camels. () was detected in 79 of 200 camels in Riyadh, an infection rate of 39.5%, and in 92 of 200 camels in Al-Qassim, an infection rate of 46%. Sequence and phylogenetic analyses revealed that the isolated was closely related to the that was detected in in Egypt and the strain ribosomal RNA gene identified from buffalo in Thailand. A BLAST search revealed that the sequences are also similar to those of from beef cattle in Thailand and to 18S ribosomal RNA from pigs in Nigeria.

摘要

我们分析了来自沙特阿拉伯利雅得和卡西姆的400头单峰骆驼的血液,以确定它们是否感染了寄生虫 spp。使用针对内部转录间隔区1(ITS1)基因的聚合酶链反应(PCR)来检测骆驼中 spp的流行情况。在利雅得的200头骆驼中有79头检测到(),感染率为39.5%,在卡西姆的200头骆驼中有92头检测到,感染率为46%。序列和系统发育分析表明,分离出的()与在埃及的()中检测到的以及从泰国水牛中鉴定出的()菌株核糖体RNA基因密切相关。BLAST搜索显示,这些序列也与泰国肉牛的()序列以及尼日利亚猪的18S核糖体RNA序列相似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f12/8074060/88209ad70759/animals-11-01149-g001.jpg

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