Metwally Dina M, Al-Turaiki Isra M, Altwaijry Najwa, Alghamdi Samia Q, Alanazi Abdullah D
Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Department of Parasitology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.
Animals (Basel). 2021 Apr 17;11(4):1149. doi: 10.3390/ani11041149.
We analyzed the blood from 400 one-humped camels, () in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite spp. Polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) gene was used to detect the prevalence of spp. in the camels. () was detected in 79 of 200 camels in Riyadh, an infection rate of 39.5%, and in 92 of 200 camels in Al-Qassim, an infection rate of 46%. Sequence and phylogenetic analyses revealed that the isolated was closely related to the that was detected in in Egypt and the strain ribosomal RNA gene identified from buffalo in Thailand. A BLAST search revealed that the sequences are also similar to those of from beef cattle in Thailand and to 18S ribosomal RNA from pigs in Nigeria.
我们分析了来自沙特阿拉伯利雅得和卡西姆的400头单峰骆驼的血液,以确定它们是否感染了寄生虫 spp。使用针对内部转录间隔区1(ITS1)基因的聚合酶链反应(PCR)来检测骆驼中 spp的流行情况。在利雅得的200头骆驼中有79头检测到(),感染率为39.5%,在卡西姆的200头骆驼中有92头检测到,感染率为46%。序列和系统发育分析表明,分离出的()与在埃及的()中检测到的以及从泰国水牛中鉴定出的()菌株核糖体RNA基因密切相关。BLAST搜索显示,这些序列也与泰国肉牛的()序列以及尼日利亚猪的18S核糖体RNA序列相似。