Riepe Tawni B, Vincent Victoria, Milano Vicki, Fetherman Eric R, Winkelman Dana L
Colorado Cooperative Fish and Wildlife Research Unit, Colorado State University, 1484 Campus Delivery, Fort Collins, CO 80523, USA.
Colorado Parks and Wildlife, Aquatic Animal Health Laboratory, 122 East Edison Street, Brush, CO 80723, USA.
Pathogens. 2021 Apr 12;10(4):460. doi: 10.3390/pathogens10040460.
Efforts to advance fish health diagnostics have been highlighted in many studies to improve the detection of pathogens in aquaculture facilities and wild fish populations. Typically, the detection of a pathogen has required sacrificing fish; however, many hatcheries have valuable and sometimes irreplaceable broodstocks, and lethal sampling is undesirable. Therefore, the development of non-lethal detection methods is a high priority. The goal of our study was to compare non-lethal sampling methods with standardized lethal kidney tissue sampling that is used to detect infections in salmonids. We collected anal, buccal, and mucus swabs (non-lethal qPCR) and kidney tissue samples (lethal DFAT) from 72 adult brook trout () reared at the Colorado Parks and Wildlife Pitkin Brood Unit and tested each sample to assess infections. Standard kidney tissue detected 1.59 times more often than mucus swabs, compared to 10.43 and 13.16 times more often than buccal or anal swabs, respectively, indicating mucus swabs were the most effective and may be a useful non-lethal method. Our study highlights the potential of non-lethal mucus swabs to sample for and suggests future studies are needed to refine this technique for use in aquaculture facilities and wild populations of inland salmonids.
许多研究都强调了推进鱼类健康诊断的努力，以改善水产养殖设施和野生鱼类种群中病原体的检测。通常，病原体的检测需要牺牲鱼类；然而，许多孵化场拥有珍贵且有时不可替代的亲鱼，致死性采样是不可取的。因此，开发非致死性检测方法是当务之急。我们研究的目的是将非致死性采样方法与用于检测鲑科鱼类感染的标准化致死性肾脏组织采样进行比较。我们从科罗拉多州公园与野生动物管理局皮特金亲鱼养殖单位饲养的72条成年溪鳟（）身上采集了肛门、口腔和黏液拭子（非致死性定量聚合酶链反应）以及肾脏组织样本（致死性直接荧光抗体检测），并对每个样本进行检测以评估感染情况。标准化肾脏组织检测出感染的频率比黏液拭子高1.59倍，分别比口腔或肛门拭子高10.43倍和13.16倍，这表明黏液拭子是最有效的，可能是一种有用的非致死性方法。我们的研究突出了非致死性黏液拭子用于采样的潜力，并建议未来需要开展研究来完善该技术，以便在水产养殖设施和内陆鲑科鱼类野生种群中使用。
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