Berby Benoit, Bichara Cynthia, Rives-Feraille Aurélie, Jumeau Fanny, Pizio Pierre Di, Sétif Véronique, Sibert Louis, Dumont Ludovic, Rondanino Chistine, Rives Nathalie
Biology of Reproduction-CECOS Laboratory, Rouen University Hospital, Normandie University, UNIROUEN, EA 4308 "Gametogenesis and Gamete Quality", F 76000 Rouen, France.
Department of Urology-Andrology, Rouen University Hospital, Normandie University, UNIROUEN, EA 4308 "Gametogenesis and Gamete Quality", F 76000 Rouen, France.
Antioxidants (Basel). 2021 Apr 12;10(4):593. doi: 10.3390/antiox10040593.
Telomere length can be influenced by reactive oxygen species (ROS) generated by lifestyle factors or environmental exposure. We sought to determine whether oxidative stress has an impact on sperm nuclear alterations, especially on chromatin organization and telomere interactions in the spermatozoa of infertile males. We performed an observational and prospective study including fifty-two males, allocated in the "case group" (30 infertile males presenting conventional semen parameter alterations) and the "control group" (22 males with normal conventional semen parameters). ROS detection was determined on spermatozoa using CellROX probes. Sperm nuclear damage was assessed using quantitative fluorescence in situ hybridization (Q-FISH) for relative telomere length and telomere number, aniline blue staining for chromatin condensation, terminal deoxynucleotidyl transferase dUTP nick-end labeling for DNA fragmentation, and FISH for aneuploidy and 8-hydroxy-2'-deoxyguanosine immunostaining for oxidative DNA damages. Infertile males had significantly increased levels of cytoplasmic ROS and chromatin condensation defects as well as a higher mean number of telomere signals per spermatozoon in comparison with controls. In addition, the mean number of sperm telomere signals were positively correlated with the percentage of spermatozoa with chromatin condensation defect. In infertile males with conventional semen parameter alterations, oxidative stress is associated with telomere interaction impairment and chromatin condensation defects.
端粒长度会受到生活方式因素或环境暴露所产生的活性氧（ROS）的影响。我们试图确定氧化应激是否会对精子核改变产生影响，尤其是对不育男性精子中的染色质组织和端粒相互作用的影响。我们进行了一项观察性前瞻性研究，纳入了52名男性，分为“病例组”（30名不育男性，其传统精液参数出现改变）和“对照组”（22名传统精液参数正常的男性）。使用CellROX探针测定精子中的ROS水平。通过定量荧光原位杂交（Q-FISH）检测相对端粒长度和端粒数量来评估精子核损伤，用苯胺蓝染色评估染色质凝聚，用末端脱氧核苷酸转移酶dUTP缺口末端标记法检测DNA片段化，用FISH检测非整倍体，并用8-羟基-2'-脱氧鸟苷免疫染色检测氧化性DNA损伤。与对照组相比，不育男性的细胞质ROS水平显著升高，染色质凝聚缺陷增加，每个精子的端粒信号平均数量也更高。此外，精子端粒信号的平均数量与染色质凝聚缺陷精子的百分比呈正相关。在传统精液参数改变的不育男性中，氧化应激与端粒相互作用受损和染色质凝聚缺陷有关。
