波兰猪种的多样性研究、个体识别和亲子关系控制的微卫星 DNA 分析。

Microsatellite DNA Analysis for Diversity Study, Individual Identification and Parentage Control in Pig Breeds in Poland.

机构信息

Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska Street 1, 32-083 Balice, Poland.

出版信息

Genes (Basel). 2021 Apr 19;12(4):595. doi: 10.3390/genes12040595.

DOI:10.3390/genes12040595

PMID:33921663

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8073418/

Abstract

Swine DNA profiling is of high importance for animal identification and parentage verification. The aim of this study was to test a set of 14 microsatellite (STR) markers recommended by ISAG for parentage verification in Polish Landrace (PL, = 900), Polish Large White (PLW, = 482), Pulawska (PUL, = 127), and Duroc pigs (DU = 108). The studied breeds showed a medium level of genetic differentiation. The average value of heterozygosity and degree of polymorphism (PIC) were above 0.5 for the studied breeds, except for the DU breed (PIC = 0.477). The population inbreeding coefficient indicates an absence of inbreeding in the studied breeds (an average value of F = 0.007). The cumulative power of discrimination for all breeds reached high values close to 1.0, while the probability of identity (P) was low, with P values ranging between 10 (for DU) and 10 (for PLW). The cumulative exclusion probability for PE and PE showed that the parentage can be confirmed with a probability of from 92.75% to 99.01% and from 99.49% to 99.97%, respectively.

摘要

猪的 DNA 图谱分析对于动物鉴定和亲子关系验证非常重要。本研究的目的是测试一套由 ISAG 推荐的 14 个微卫星（STR）标记，用于波兰长白猪（PL，n = 900）、波兰大白猪（PLW，n = 482）、波兰猪（PUL，n = 127）和杜洛克猪（DU，n = 108）的亲子关系验证。研究的品种表现出中等水平的遗传分化。除了 DU 品种（PIC = 0.477）外，研究品种的平均杂合度和多态性程度（PIC）均高于 0.5。种群近交系数表明研究品种不存在近交（平均值 F = 0.007）。所有品种的累积鉴别力接近 1.0，达到了很高的值，而个体识别概率（P）较低，范围在 10（对于 DU）到 10（对于 PLW）之间。PE 和 PE 的累积排除概率表明亲子关系的确认概率分别为 92.75%至 99.01%和 99.49%至 99.97%。