Nechtelberger D, Kaltwasser C, Stur I, Meyer J N, Brem G, Mueller M, Mueller S
Institute for Animal Breeding and Genetics, Vienna University of Veterinary Medicine, Austria.
Anim Biotechnol. 2001 Nov;12(2):141-4. doi: 10.1081/ABIO-100108340.
We present an efficient parentage control for pigs based on ten polymorphic microsatellite markers analyzed in a single PCR reaction. Assuming one known parent ("paternity control"), combined exclusion probabilities (CEPs) ranged from 99.18% (Landrace), 99.74% (Piétrain) to 99.76% (Large White) for the most important Austrian breeds. Assuming a known parent-pair ("parentage control", e.g. a substituted offspring), the CEP of the 10-plex PCR increased to 99.97% (Landrace) and 99.99% (Piétrain and Large White). We developed an additional standby battery of 5 markers, which might be applied in those cases, where the CEP of the 10-plex PCR is not sufficient. Therefore an automated, cost and time reduced genotype analysis for pigs is available.
我们基于在单一聚合酶链反应（PCR）中分析的十个多态性微卫星标记，提出了一种高效的猪亲子鉴定方法。假设一个已知亲本（“父系鉴定”），对于奥地利最重要的几个品种，联合排除概率（CEP）范围从长白猪的99.18%、皮特兰猪的99.74%到大白猪的99.76%。假设已知亲本对（“亲权鉴定”，例如替代后代），十重PCR的CEP增加到长白猪的99.97%以及皮特兰猪和大白猪的99.99%。我们开发了另外一组由5个标记组成的备用组合，可应用于十重PCR的CEP不够的情况。因此，可实现猪的自动化、成本降低且耗时减少的基因型分析。
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