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病毒样颗粒免疫诱导的被动免疫和抗体反应

Passive Immunity and Antibody Response Induced by VLP Immunization.

作者信息

Kang Hae-Ji, Kim Min-Ju, Chu Ki-Back, Lee Su-Hwa, Moon Eun-Kyung, Quan Fu-Shi

机构信息

Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Korea.

Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul 02447, Korea.

出版信息

Vaccines (Basel). 2021 Apr 23;9(5):425. doi: 10.3390/vaccines9050425.

DOI:10.3390/vaccines9050425
PMID:33922808
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8146287/
Abstract

Passive immunity can provide immediate protection against infectious pathogens. To date, only a few studies have investigated the effect of passive immunization against , and the use of immune sera acquired from VLP-vaccinated mice for passive immunity assessment remains unreported. In this study, immune sera were produced by a single immunization with virus-like particles (VLPs) expressing the inner membrane complex (IMC), rhoptry protein 18 (ROP18), and microneme protein 8 (MIC8) of , with or without a CpG-ODN adjuvant. The passive immunization of immune sera conferred protection in mice, as indicated by their potent parasite-specific antibody response, lessened brain cyst counts, lower bodyweight loss, and enhanced survival. In order to confirm that the immune sera of the VLP-immunized mice were truly protective, the antibody responses and other immunological parameters were measured in the VLP-immunized mice. We found that VLP immunization induced higher levels of parasite-specific IgG, IgG subclass, and IgM antibody responses in the sera and intestines than in the controls. Enhanced Th1 and Th2-associated cytokines in the spleen, diminished brain cyst counts, and lessened body weight loss were found following ME49 challenge infection. These results suggest that passive immunization with the immune sera acquired from VLP-vaccinated mice can confer adequate protection against infection.

摘要

被动免疫可以提供针对传染性病原体的即时保护。迄今为止，仅有少数研究调查了被动免疫对[病原体名称未明确]的影响，且利用从接种病毒样颗粒（VLP）的小鼠获得的免疫血清进行被动免疫评估的情况尚未见报道。在本研究中，通过单次免疫表达[病原体名称未明确]内膜复合物（IMC）、棒状体蛋白18（ROP18）和微线体蛋白8（MIC8）的病毒样颗粒（VLP）来制备免疫血清，免疫时添加或不添加CpG - ODN佐剂。免疫血清的被动免疫在小鼠中赋予了保护作用，表现为其强大的寄生虫特异性抗体反应、脑囊肿数量减少、体重减轻程度降低以及存活率提高。为了确认VLP免疫小鼠的免疫血清确实具有保护作用，对VLP免疫小鼠的抗体反应和其他免疫参数进行了测量。我们发现，与对照组相比，VLP免疫诱导血清和肠道中更高水平的寄生虫特异性IgG、IgG亚类和IgM抗体反应。在ME49攻击感染后，脾脏中Th1和Th2相关细胞因子增强，脑囊肿数量减少，体重减轻程度减轻。这些结果表明，用从VLP免疫小鼠获得的免疫血清进行被动免疫可以对[病原体名称未明确]感染提供充分的保护。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/e2e6b7f7b598/vaccines-09-00425-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/08251d4f8d32/vaccines-09-00425-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/6d3c6d882979/vaccines-09-00425-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/f8f5163e24c2/vaccines-09-00425-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/25545a78dcc8/vaccines-09-00425-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/48c09ed38548/vaccines-09-00425-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/713d318e4736/vaccines-09-00425-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/e2e6b7f7b598/vaccines-09-00425-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/08251d4f8d32/vaccines-09-00425-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/6d3c6d882979/vaccines-09-00425-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/f8f5163e24c2/vaccines-09-00425-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/25545a78dcc8/vaccines-09-00425-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/48c09ed38548/vaccines-09-00425-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/713d318e4736/vaccines-09-00425-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cfd/8146287/e2e6b7f7b598/vaccines-09-00425-g007.jpg

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2
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Pharmaceutics. 2020 Oct 19;12(10):989. doi: 10.3390/pharmaceutics12100989.
3
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Parasite Immunol. 2021 Jan;43(1):e12799. doi: 10.1111/pim.12799. Epub 2020 Oct 26.
4
A chimeric yellow fever-Zika virus vaccine candidate fully protects against yellow fever virus infection in mice.嵌合黄热病-寨卡病毒疫苗候选物可完全保护小鼠免受黄热病病毒感染。
Emerg Microbes Infect. 2020 Mar 2;9(1):520-533. doi: 10.1080/22221751.2020.1730709. eCollection 2020.
5
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6
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