Department of Biomedical Engineering, National Yang-Ming University, Taipei 11221, Taiwan.
Division of Endocrinology and Metabolism, Department of Internal Medicine, Tri-Service General Hospital Songshan Branch, National Defense Medical Center, Taipei 11490, Taiwan.
Sensors (Basel). 2021 Apr 25;21(9):3017. doi: 10.3390/s21093017.
Electric cell-substrate impedance sensing (ECIS) has been used as a real-time impedance-based method to quantify cell behavior in tissue culture. The method is capable of measuring both the resistance and capacitance of a cell-covered microelectrode at various AC frequencies. In this study, we demonstrate the application of high-frequency capacitance measurement (f = 40 or 64 kHz) for the sensitive detection of both the micromotion and wound-healing migration of human mesenchymal stem cells (hMSCs). Impedance measurements of cell-covered electrodes upon the challenge of various concentrations of carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), from 0.1 to 30 μM, were conducted using ECIS. FCCP is an uncoupler of mitochondrial oxidative phosphorylation (OXPHOS), thereby reducing mitochondrial ATP production. By numerically analyzing the time-series capacitance data, a dose-dependent decrease in hMSC micromotion and wound-healing migration was observed, and the effect was significantly detected at levels as low as 0.1 μM. While most reported works with ECIS use the resistance/impedance time series, our results suggest the potential use of high-frequency capacitance time series for assessing migratory cell behavior such as micromotion and wound-healing migration.
电细胞-基底阻抗感应（ECIS）已被用作一种实时基于阻抗的方法来量化组织培养中的细胞行为。该方法能够在各种交流频率下测量覆盖微电极的细胞的电阻和电容。在这项研究中，我们展示了高频电容测量（f = 40 或 64 kHz）在敏感检测人间充质干细胞（hMSC）的微运动和创伤愈合迁移中的应用。使用 ECIS 对覆盖细胞的电极进行阻抗测量，以应对各种浓度的羰基氰化物 4-（三氟甲氧基）苯腙（FCCP），从 0.1 到 30 μM。FCCP 是线粒体氧化磷酸化（OXPHOS）的解偶联剂，从而减少线粒体 ATP 的产生。通过对时间序列电容数据进行数值分析，观察到 hMSC 微运动和创伤愈合迁移的剂量依赖性下降，并且在低至 0.1 μM 的水平下就可以显著检测到这种作用。虽然 ECIS 的大多数报道工作都使用电阻/阻抗时间序列，但我们的结果表明，高频电容时间序列可能用于评估迁移细胞行为，如微运动和创伤愈合迁移。
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