Department of Systems Biotechnology, Chung-Ang University, Gyeonggi 17546, Korea.
Department of Orthopedic Surgery and Nano-Based Disease Control Institute, Korea University Guro Hospital, #148, Gurodong-ro, Guro-gu, Seoul 08308, Korea.
Int J Mol Sci. 2021 Apr 28;22(9):4614. doi: 10.3390/ijms22094614.
Calcium carbonate (CaCO)-based materials have received notable attention for biomedical applications owing to their safety and beneficial characteristics, such as pH sensitivity, carbon dioxide (CO) gas generation, and antacid properties. Herein, to additionally incorporate antioxidant and anti-inflammatory functions, we prepared tannylated CaCO (TA-CaCO) materials using a simple reaction between tannic acid (TA), calcium (Ca), and carbonate (CO) ions. TA-CaCO synthesized at a molar ratio of 1:75 (TA:calcium chloride (CaCl)/sodium carbonate (NaCO)) showed 3-6 μm particles, comprising small nanoparticles in a size range of 17-41 nm. The TA-CaCO materials could efficiently neutralize the acid solution and scavenge free radicals. In addition, these materials could significantly reduce the mRNA levels of pro-inflammatory factors and intracellular reactive oxygen species, and protect chondrocytes from toxic hydrogen peroxide conditions. Thus, in addition to their antacid property, the prepared TA-CaCO materials exert excellent antioxidant and anti-inflammatory effects through the introduction of TA molecules. Therefore, TA-CaCO materials can potentially be used to treat inflammatory cells or diseases.
碳酸钙（CaCO）基材料由于其安全性和有益特性，如 pH 敏感性、二氧化碳（CO）气体生成和抗酸特性，在生物医学应用中受到了广泛关注。在此，为了额外结合抗氧化和抗炎功能，我们使用单宁酸（TA）、钙（Ca）和碳酸根（CO）离子之间的简单反应制备了单宁酰化 CaCO（TA-CaCO）材料。在摩尔比为 1:75（TA：氯化钙（CaCl）/碳酸钠（NaCO））的条件下合成的 TA-CaCO 显示出 3-6μm 的颗粒，包含 17-41nm 范围内的小纳米颗粒。TA-CaCO 材料能够有效地中和酸性溶液并清除自由基。此外，这些材料还可以显著降低促炎因子和细胞内活性氧物质的 mRNA 水平，并保护软骨细胞免受有毒过氧化氢条件的影响。因此，除了抗酸特性外，通过引入 TA 分子，制备的 TA-CaCO 材料还表现出优异的抗氧化和抗炎作用。因此，TA-CaCO 材料可用于治疗炎症细胞或疾病。
