National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Microelement Research Centre, Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2021 Apr 28;22(9):4625. doi: 10.3390/ijms22094625.
Low phosphorus (P) availability is one of the major constraints to plant growth, particularly in acidic soils. A possible mechanism for enhancing the use of sparsely soluble P forms is the secretion of malate in plants by the aluminum-activated malate transporter (ALMT) gene family. Despite its significance in plant biology, the identification of the gene family in oilseed rape (; ), an allotetraploid crop, is unveiled. Herein, we performed genome-wide identification and characterization of in , determined their gene expression in different tissues and monitored transcriptional regulation of in the roots and leaves at both a sufficient and a deficient P supply. Thirty-nine genes were identified and were clustered into five branches in the phylogenetic tree based on protein sequences. Collinearity analysis revealed that most of the genes shared syntenic relationships among members in , which suggested that whole-genome duplication (polyploidy) played a major driving force for evolution in addition to segmental duplication. RNA-seq analyses showed that most genes were preferentially expressed in root and leaf tissues. Among them, the expression of , , , , , , , , , , were significantly up-regulated in roots and leaf at a P deficient supply. The current study analyzes the evolution and the expression of the ALMT family in , which will help in further research on their role in the enhancement of soil P availability by secretion of organic acids.
低磷（P）供应是植物生长的主要限制因素之一，特别是在酸性土壤中。增强对稀疏可溶性 P 形态利用的一种可能机制是通过铝激活的苹果酸转运蛋白（ALMT）基因家族在植物中分泌苹果酸。尽管它在植物生物学中具有重要意义，但在异源四倍体作物油菜（）中，尚未揭示其基因家族的鉴定。在此，我们对油菜中的进行了全基因组鉴定和特征分析，确定了它们在不同组织中的基因表达，并在充分和缺乏 P 供应的情况下监测了根和叶中基因的转录调控。鉴定出 39 个基因，并根据蛋白质序列在系统发育树上聚类为五个分支。共线性分析表明，大多数基因在油菜中成员之间具有共线性关系，这表明全基因组复制（多倍体）除了片段复制之外，还是基因进化的主要驱动力。RNA-seq 分析表明，大多数基因在根和叶组织中优先表达。其中，在 P 缺乏供应时，、、、、、、、、、、的表达在油菜根和叶中显著上调。本研究分析了 ALMT 家族在油菜中的进化和表达，这将有助于进一步研究其通过分泌有机酸增强土壤 P 有效性的作用。
