Jones D S, Flint A P
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge.
J Endocrinol. 1988 Jun;117(3):409-14. doi: 10.1677/joe.0.1170409.
Concentrations of oxytocin in corpora lutea from pregnant and non-pregnant sheep rose during luteinization to peak between days 5 and 9 after oestrus before falling to low levels on days 12 and 14. Concentrations of the 608-base mRNA encoding the oxytocin-neurophysin prohormone, measured by Northern blotting using a cRNA probe, were highest before day 5, and fell to low levels by day 9. The decline in oxytocin during the second half of the oestrus cycle and in pregnancy could therefore be accounted for by reduced luteal concentrations of oxytocin-neurophysin mRNA. The rate of decline in prohormone mRNA concentration between days 3 and 16 followed first order kinetics, suggesting that gene expression ceased early in the cycle. The lag between peak oxytocin-neurophysin mRNA and peak oxytocin concentrations may represent the time taken for post-translational processing of the prohormone.
怀孕和未怀孕绵羊黄体中的催产素浓度在黄体化过程中上升，在发情后第5至9天达到峰值，然后在第12和14天降至低水平。使用cRNA探针通过Northern印迹法测量的编码催产素-神经垂体激素原激素的608个碱基的mRNA浓度在第5天之前最高，并在第9天降至低水平。因此，发情周期后半期和怀孕期间催产素的下降可能是由于黄体中催产素-神经垂体激素mRNA浓度降低所致。前激素mRNA浓度在第3天至16天之间的下降速率遵循一级动力学，表明基因表达在周期早期就停止了。催产素-神经垂体激素mRNA峰值与催产素浓度峰值之间的延迟可能代表前激素翻译后加工所需的时间。
