Lebordais Marc, Venel Zélie, Gigault Julien, Langlois Valerie S, Baudrimont Magalie
Université de Bordeaux, CNRS, UMR EPOC 5805, Place du Dr Peyneau, 33120 Arcachon, France.
Centre Eau Terre Environnement, Institut National de la Recherche Scientifique (INRS), 490 rue de la Couronne, Québec City, QC G1K 9A9, Canada.
Nanomaterials (Basel). 2021 Apr 28;11(5):1151. doi: 10.3390/nano11051151.
Nanoplastics (NPs) are anthropogenic contaminants that raise concern, as they cross biological barriers. Metals' adsorption on NPs' surface also carries ecotoxicological risks to aquatic organisms. This study focuses on the impacts of three distinct NPs on the Caribbean oyster through dietary exposure. As such, marine microalgae Tisochrysis lutea were exposed to environmentally weathered mixed NPs from Guadeloupe (NPG), crushed pristine polystyrene nanoparticles (PSC), and carboxylated polystyrene nanoparticles of latex (PSL). Oysters were fed with NP-T. lutea at 10 and 100 µg L, concentrations considered environmentally relevant, combined or not with 1 mg L pentoxide arsenic (As) in water. We investigated key gene expression in I. alatus' gills and visceral mass. NP treatments revealed significant induction of cat and sod1 in gills and gapdh and sod1 in visceral mass. As treatment significantly induced sod1 expression in gills, but once combined with any of the NPs at both concentrations, basal mRNA levels were observed. Similarly, PSL treatment at 100 µg L that significantly induced cat expression in gills or sod1 in visceral mass showed repressed mRNA levels when combined with As (reduction of 2222% and 34%, respectively, compared to the control). This study suggested a protective effect of the interaction between NPs and As, possibly by decreasing both contaminants' surface reactivity.
纳米塑料（NPs）是人为产生的污染物，因其能跨越生物屏障而引发关注。金属吸附在纳米塑料表面也会给水生生物带来生态毒理学风险。本研究聚焦于三种不同的纳米塑料通过饮食暴露对加勒比牡蛎产生的影响。具体而言，海洋微藻金藻（Tisochrysis lutea）被暴露于来自瓜德罗普岛的环境风化混合纳米塑料（NPG）、粉碎的原始聚苯乙烯纳米颗粒（PSC）以及乳胶羧化聚苯乙烯纳米颗粒（PSL）。牡蛎以每升10微克和100微克的纳米塑料 - 金藻为食，这两个浓度被认为与环境相关，水中还分别添加或不添加每升1毫克的五氧化二砷（As）。我们研究了加勒比牡蛎鳃和内脏团中的关键基因表达。纳米塑料处理显示，鳃中的过氧化氢酶（cat）和超氧化物歧化酶1（sod1）以及内脏团中的甘油醛 - 3 - 磷酸脱氢酶（gapdh）和超氧化物歧化酶1有显著诱导。砷处理显著诱导了鳃中超氧化物歧化酶1的表达，但当与两种浓度的任何一种纳米塑料组合时，观察到基础mRNA水平。同样，每升100微克的PSL处理显著诱导了鳃中的过氧化氢酶表达或内脏团中的超氧化物歧化酶1表达，但与砷组合时，mRNA水平受到抑制（与对照相比分别降低了2222%和34%）。本研究表明纳米塑料和砷之间的相互作用具有保护作用，可能是通过降低两种污染物的表面反应性实现的。
