Whanger P D, Butler J A
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331.
J Nutr. 1988 Jul;118(7):846-52. doi: 10.1093/jn/118.7.846.
Weanling rats were fed a basal diet or this diet plus 0.2, 1.0, 2.0 or 4.0 mg/kg selenium (Se) as either selenite or selenomethionine (SeM). Except at the 0.2 mg/kg Se level, Se accumulated in all tissues at higher levels when SeM was fed than when selenite was given, and the magnitude of difference became more pronounced with increasing levels of dietary Se. This was particularly true for muscle and brain. Se levels in whole blood, testes, kidney and lungs were not significantly different between rats fed 0.2 mg/kg Se as selenite or as SeM, but the Se levels in liver, muscle and brain were higher in rats fed SeM. Although the tissue Se concentrations differed markedly, there were no differences in the glutathione peroxidase (GPX) activity in tissues of rats fed SeM rather than selenite. The percentage of Se associated with GPX was lower in all tissues from rats fed SeM than in those from rats fed selenite. These results indicate that the chemical forms of dietary Se can have a marked influence on biological responses, including bioavailability of dietary Se.
给断乳大鼠喂食基础日粮，或在基础日粮中添加0.2、1.0、2.0或4.0毫克/千克的硒（Se），硒的形式为亚硒酸盐或硒代蛋氨酸（SeM）。除了0.2毫克/千克硒水平外，当喂食SeM时，所有组织中硒的积累水平高于喂食亚硒酸盐时，并且随着日粮硒水平的增加，差异程度变得更加明显。肌肉和大脑尤其如此。喂食0.2毫克/千克亚硒酸盐或SeM的大鼠，全血、睾丸、肾脏和肺中的硒水平没有显著差异，但喂食SeM的大鼠肝脏、肌肉和大脑中的硒水平更高。尽管组织中的硒浓度差异显著，但喂食SeM而非亚硒酸盐的大鼠组织中的谷胱甘肽过氧化物酶（GPX）活性没有差异。喂食SeM的大鼠所有组织中与GPX结合的硒百分比低于喂食亚硒酸盐的大鼠。这些结果表明，日粮硒的化学形式可对生物学反应产生显著影响，包括日粮硒的生物利用度。
