Cell Lysis Directed by SulA in Response to DNA Damage in .

The SOS response is induced upon DNA damage and the inhibition of Z ring formation by the product of the gene, which is one of the LexA-regulated genes, allows time for repair of damaged DNA. On the other hand, severely DNA-damaged cells are eliminated from cell populations. Overexpression of leads to cell lysis, suggesting SulA eliminates cells with unrepaired damaged DNA. Transcriptome analysis revealed that overexpression of leads to up-regulation of numerous genes, including Deletion of markedly reduced the extent of cell lysis by overexpression and overexpression alone led to cell lysis. Further experiments on the SoxS regulon suggested that LpxC is a main player downstream from SoxS. These findings suggested the SulA-dependent cell lysis (SDCL) cascade as follows: SulA→SoxS→LpxC. Other tests showed that the SDCL cascade pathway does not overlap with the apoptosis-like and cell death pathways.