Yazıcılar Büşra, Jannati Gholamreza, Bezirganoglu Ismail
Department of Molecular Biology and Genetics, Erzurum Technical University, 25050, Erzurum, Turkey.
Department of Agronomy, Faculty of Agriculture, Ataturk University, Erzurum, Turkey.
J Genet Eng Biotechnol. 2021 Apr 29;19(1):59. doi: 10.1186/s43141-021-00159-6.
Alfalfa (Medicago sativa L.) is a perennial plant, which is high in nutritional value and resistant to environmental conditions, and it is one of most frequently preferred feed crop among the leguminous family. In this study, it was aimed to determine the genetic diversity of some alfalfa ecotypes and their varieties by DNA, protein, nucleus, and chromosome counts. The genetic distance between the populations of control (M. truncatula), five different cultivars (Alsancak, Bilensoy, Iside, Plato, Bilensoy82), and three different ecotypes (Erzurum, Muş, and Konya) was investigated by cytogenetic analysis, flow cytometry, simple sequence repeats (SSR), and SDS PAGE techniques.
Cytogenetic analysis of these tested plants has verified the existence of expected levels such as diploid, triploid, and tetraploid as well as aneuploid (2n = 4x = 30) plants. Flow cytometry analysis have displayed that all of tested plants were tetraploid, whereas cytological analysis had either diploid, triploid, or tetraploid. Genetic diversity dendrogram was created using Erzurum, Muş, Konya, Bilensoy82, Alsancak, and Plato varieties. The Iside and Bilensoy were found to be morphogenetic in relationship. Our control plant, M. truncatula, did not have a similarity relationship with other ecotypes and cultivars. The total numbers of protein bands differed among tested plants from 140 kDA to 25 kDa.
This paper first reports on the genetic variation of Turkish alfalfa plants by using detailed analysis techniques. This work provides important findings for the classification, conservation, and innovation of alfalfa germplasm resources.
紫花苜蓿（Medicago sativa L.）是一种多年生植物，营养价值高且耐环境条件，是豆科中最常被选用的饲料作物之一。本研究旨在通过DNA、蛋白质、细胞核和染色体计数来确定一些紫花苜蓿生态型及其品种的遗传多样性。通过细胞遗传学分析、流式细胞术、简单序列重复（SSR）和SDS-PAGE技术研究了对照（蒺藜苜蓿）、五个不同品种（阿尔桑卡克、比伦索伊、伊西德、柏拉图、比伦索伊82）和三个不同生态型（埃尔祖鲁姆、穆什和科尼亚）群体之间的遗传距离。
对这些受试植物的细胞遗传学分析证实了二倍体、三倍体和四倍体以及非整倍体（2n = 4x = 30）植物等预期水平的存在。流式细胞术分析表明所有受试植物均为四倍体，而细胞学分析则显示有二倍体、三倍体或四倍体。使用埃尔祖鲁姆、穆什、科尼亚、比伦索伊82、阿尔桑卡克和柏拉图品种构建了遗传多样性树状图。发现伊西德和比伦索伊在形态发生上有关系。我们的对照植物蒺藜苜蓿与其他生态型和品种没有相似关系。受试植物中蛋白质条带的总数在140 kDa至25 kDa之间有所不同。
本文首次通过使用详细的分析技术报道了土耳其紫花苜蓿植物的遗传变异。这项工作为紫花苜蓿种质资源的分类、保护和创新提供了重要发现。
