Keck Graduate Institute, The Claremont Colleges, Claremont, CA, 91711, USA.
Cardea Bio Inc., 8969 Kenamar Dr. Suite 104, San Diego, CA, 92121, USA.
Adv Biol (Weinh). 2021 Jul;5(7):e2000594. doi: 10.1002/adbi.202000594. Epub 2021 Apr 30.
Increasing access to modern clinical practices concomitantly extends lifespan, ironically revealing new classes of degenerative and inflammatory diseases of later years. Here, an electronic graphene field-effect transistor (gFET) is reported, termed EV-chip, for label-free, rapid identification and quantification of exosomes (EV) associated with aging through specific surface markers, CD63 and CD151. Studies suggest that blood-derived exosomes carry specific biomolecules that can be used toward diagnostic applications of age and health. However, to observe improvements in patient outcomes, earlier detection at the point-of-care (POC) is required. Unfortunately, conventional techniques and other electronic-based platforms for exosome sensing are burdensome and inept for the POC distinction of aged blood factors. It is shown that EV-chip can quantitatively detect purified exosomes from plasma, with a limit of detection (LOD) of 2 × 10 particles mL and a limit of quantification (LOQ) of 6 × 10 particles mL . The sensitivity and compact electronics of the EV-chip improves upon previously published electronic biosensors, making it ideal for a physician's office or a simple biological laboratory. The sensitivity, selectivity, and portability of the EV-chip demonstrate the potential of the biosensor as a powerful point-of-care diagnostic and prognostic tool for age-related diseases.
随着现代临床实践的普及，人们的寿命延长了，但讽刺的是，这也揭示了新的一类退行性和炎症性疾病。在这里，我们报告了一种称为 EV-chip 的电子石墨烯场效应晶体管（gFET），它可通过特定的表面标志物 CD63 和 CD151 对与衰老相关的外泌体（EV）进行无标记、快速的识别和定量。研究表明，血液衍生的外泌体携带特定的生物分子，可用于年龄和健康的诊断应用。然而，为了观察到患者预后的改善，需要在护理点（POC）更早地进行检测。不幸的是，用于外泌体传感的常规技术和其他基于电子的平台对于 POC 区分老化的血液因素来说是繁琐和不切实际的。研究表明，EV-chip 可以定量检测来自血浆的纯化外泌体，检测限（LOD）为 2×10 个颗粒/mL，定量限（LOQ）为 6×10 个颗粒/mL。EV-chip 的灵敏度和紧凑的电子器件优于以前发表的电子生物传感器，使其成为医生办公室或简单生物实验室的理想选择。EV-chip 的灵敏度、选择性和便携性表明，该生物传感器作为一种强大的即时诊断和预后工具，具有用于与年龄相关的疾病的潜力。
