Oral and Maxillofacial Pathology Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Mol Biol Rep. 2021 Apr;48(4):3223-3235. doi: 10.1007/s11033-021-06341-w. Epub 2021 Apr 30.
Aflibercept and arsenic trioxide drugs apply a cytotoxic effect on some human cancer cell lines. However, no more study has followed the effects of both drugs, especially arsenic trioxide, on oral squamous cell carcinoma (OCC). We used three OCC lines as a model to show the effect of these drugs on the genetically complex disease and investigate its targeted therapy. In this study, three human OCC cell lines were used from different patients. We treated cell lines with both medications to detect the effect and relevant molecular basis. First, methyl thiazolyl tetrazolium (MTT) assay was performed to detect the cytotoxicity effect and cell growth. Second, flow cytometry, gene and protein expression were performed to evaluate the anti-angiogenic effect on OCC lines. Next apoptosis was analyzed by flow cytometry. Finally, clonogenesis capacity and cell migration were assessed by colony formation assay and wound healing, respectively. Aflibercept had no cytotoxic effect on the three OCC cell lines but decreased cell growth rate. Arsenic trioxide had a significant cytotoxic effect on three cell lines. Our results demonstrated that both drugs significantly decreased endoglin, VEGFA, and VEGFB expression. In addition, Migration and colony formation assays confirmed that these drugs have significant anti-proliferative and anti-migration effect on oral carcinoma cells. These results revealed that both medications might be a potential drug for the management of oral cancer patients.
阿柏西普和三氧化二砷药物对一些人类癌细胞系具有细胞毒性作用。然而，没有更多的研究关注这两种药物（尤其是三氧化二砷）对口腔鳞状细胞癌（OCC）的影响。我们使用三种 OCC 细胞系作为模型，以展示这些药物对这种遗传复杂疾病的影响，并研究其靶向治疗。在这项研究中，我们使用了来自不同患者的三种人类 OCC 细胞系。我们用这两种药物处理细胞系，以检测其效果及其相关的分子基础。首先，进行甲基噻唑基四唑（MTT）测定以检测细胞毒性作用和细胞生长。其次，通过流式细胞术、基因和蛋白质表达来评估对 OCC 细胞系的抗血管生成作用。接下来，通过流式细胞术分析细胞凋亡。最后，通过集落形成测定和划痕愈合分别评估克隆形成能力和细胞迁移。阿柏西普对三种 OCC 细胞系没有细胞毒性作用，但降低了细胞生长速率。三氧化二砷对三种细胞系均有显著的细胞毒性作用。我们的结果表明，这两种药物均显著降低了内胚层蛋白、VEGFA 和 VEGFB 的表达。此外，迁移和集落形成测定证实，这些药物对口腔癌细胞具有显著的增殖抑制和迁移抑制作用。这些结果表明，这两种药物可能是治疗口腔癌患者的潜在药物。
