Cellular Protein Chemistry, Bijvoet Centre for Biomolecular Research, Utrecht University, Utrecht, The Netherlands; Science for Life, Utrecht University, Utrecht, The Netherlands.
Cellular Protein Chemistry, Bijvoet Centre for Biomolecular Research, Utrecht University, Utrecht, The Netherlands; Science for Life, Utrecht University, Utrecht, The Netherlands.
J Biol Chem. 2021 Jan-Jun;296:100717. doi: 10.1016/j.jbc.2021.100717. Epub 2021 Apr 27.
Heat shock 70 kDa protein (Hsp70) chaperones play a crucial role in the biogenesis of tail-anchored proteins (TAs), starting a downstream cascade to the endoplasmic reticulum (ER) via the guided-entry-of-tail-anchored protein (GET) pathway. J-domain proteins (JDPs) are generally known to assist Hsp70s, but their specific role in TA targeting remains unclear. Cho et al. now identify two separate functions for JDPs in the process, in the initial capture of the TA and the transfer into the GET pathway. These data suggest that several Hsp70 cycles could be involved at distinct steps during protein maturation.
热休克 70 kDa 蛋白 (Hsp70) 伴侣在尾部锚定蛋白 (TA) 的生物发生中起着至关重要的作用，通过引导进入尾部锚定蛋白 (GET) 途径，启动了内质网 (ER) 的下游级联反应。众所周知，J 结构域蛋白 (JDPs) 通常可以协助 Hsp70，但它们在 TA 靶向中的具体作用仍不清楚。Cho 等人。现在在这个过程中确定了 JDPs 的两个独立功能，即 TA 的初始捕获和进入 GET 途径的转移。这些数据表明，在蛋白质成熟的不同步骤中可能涉及多个 Hsp70 循环。
