Benedetti A, Jezequel A M, Orlandi F
Postgraduate School of Gastroenterology, University of Ancona, School of Medicine, Italy.
Liver. 1988 Jun;8(3):172-7. doi: 10.1111/j.1600-0676.1988.tb00987.x.
The aim of the present study was to determine if data on the number and acinar distribution of apoptotic bodies (AB) in normal liver could help in the understanding of cell kinetics in the liver, and the mechanism of early ethanol-induced liver damage. Normal male Sprague-Dawley rats were studied. They had free access to Purina chow and drinking water. Ethanol-treated rats received the drug at increasing concentration in drinking water for 5 weeks. The following parameters were measured: number of AB in the lobule, topographical localization, distance from terminal hepatic veins (THV), i.e. row of hepatocytes concerned, H1 being the closest to the THV. The results show that AB are rare in the normal liver and are always observed in zone 3, next to the THV. Of 149 THV examined, 56 showed one associated AB, exceptionally two. 74% of the AB were confined to the first row of hepatocytes (H1), 21% to H2, 4% to H3, and 1% to H4. In ethanol-treated rats the mean number of AB was 2 or 3 for each THV. 42% were found in H1, 32% in H2, 15% in H3, 7% in H4, and 4% in H5. The data show that AB are not randomly dispersed in normal liver but show a preferential acinar distribution. In addition, most AB are located in the row of liver cells immediately adjacent to the THV. If apoptosis is indeed an expression of physiological cell renewal, these findings support the concept that zone 3 contains older hepatocytes.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究的目的是确定正常肝脏中凋亡小体（AB）的数量和腺泡分布数据是否有助于理解肝脏中的细胞动力学以及早期乙醇诱导的肝损伤机制。对正常雄性Sprague-Dawley大鼠进行了研究。它们可自由获取普瑞纳饲料和饮用水。乙醇处理的大鼠在饮用水中接受浓度递增的药物处理5周。测量了以下参数：小叶中AB的数量、地形定位、距终末肝静脉（THV）的距离，即相关肝细胞排，H1是最靠近THV的一排。结果显示，AB在正常肝脏中很少见，且总是在3区，紧邻THV处观察到。在检查的149个THV中，56个显示有1个相关的AB，极少数有2个。74%的AB局限于第一排肝细胞（H1），21%在H2，4%在H3，1%在H4。在乙醇处理的大鼠中，每个THV的AB平均数为2或3个。42%在H1中发现，32%在H2中，15%在H3中，7%在H4中，4%在H5中。数据表明，AB在正常肝脏中并非随机分布，而是呈现出优先的腺泡分布。此外，大多数AB位于紧邻THV的肝细胞排中。如果凋亡确实是生理性细胞更新的一种表现，这些发现支持了3区含有较老肝细胞的概念。（摘要截短于250字）
