Centre for Functional Ecology (CFE), Department of Life Sciences, University of Coimbra, Coimbra, Portugal.
Faculty of Biology Alexandru Ioan Cuza, University of Iași, Romania.
J Hazard Mater. 2021 Jul 5;413:125443. doi: 10.1016/j.jhazmat.2021.125443. Epub 2021 Feb 16.
The environmental effects of nanoplastics-NPLs have been addressed mainly through short-term exposures to a few types of polymers, neglecting other NPLs that are economically relevant like polymethylmethacrylate - PMMA. This work aimed to assess long-term effects of PMMA-NPLs on the marine primary consumer Brachionus plicatilis, evaluating the influence of different exposure routes (waterborne, foodborne and both) and food items (Nannochloropsis gaditana and Tetraselmis chuii). Rotifers were 21 days exposed to: a) control, with clean medium and food-CTR; b) contaminated medium (8.1 mg PMMA-NPLs/L) and clean algae-MC; c) clean medium and contaminated algae (pre-incubated for 96 h on 8.1 mg PMMA-NPLs/L)-AC; and, d) contaminated medium and algae-MC/AC. Mortality (lx), total number of organisms (TN), fecundity (mx), populational growth rate (r), generational time (gt), and feeding rates were assessed. Effects on r and mx were found after 21 days. Organisms from AC had higher r than MC. MC/AC organisms performed better than control in all endpoints. Overall organisms fed with N. gaditana had higher TN, m and r than those fed with T. chuii. In the AC treatments, rotifers fed with N. gaditana had higher m. Results highlight that exposure route and food type may modulate NPLs' effects, supporting the need for standardization of assays.
纳米塑料（NPLs）的环境影响主要通过对几种类型聚合物的短期暴露来研究，而忽略了其他经济相关的 NPLs，如聚甲基丙烯酸甲酯（PMMA）。本研究旨在评估 PMMA-NPLs 对海洋初级消费者褶皱臂尾轮虫的长期影响，评估不同暴露途径（水相、食物相和两者兼有）和食物种类（盐藻和球等鞭金藻）的影响。轮虫在 21 天内暴露于以下环境中：a）对照组，使用清洁介质和食物对照（CTR）；b）污染介质（8.1mg PMMA-NPLs/L）和清洁藻类-对照（MC）；c）清洁介质和受污染的藻类（在 8.1mg PMMA-NPLs/L 下预先孵育 96 小时）-对照加污染物（AC）；以及 d）污染介质和藻类-MC/AC。评估死亡率（lx）、总生物量（TN）、繁殖力（mx）、种群增长率（r）、世代时间（gt）和摄食率。在 21 天后发现 r 和 mx 受到影响。AC 中的生物具有比 MC 更高的 r。MC/AC 中的生物在所有终点都比对照表现更好。总的来说，用盐藻喂养的生物具有比用球等鞭金藻喂养的生物更高的 TN、m 和 r。在 AC 处理中，用盐藻喂养的轮虫具有更高的 m。研究结果表明，暴露途径和食物类型可能会调节 NPLs 的影响，这支持了对测定方法进行标准化的需要。
