State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, Gansu, China.
Anal Bioanal Chem. 2021 Jun;413(15):3925-3932. doi: 10.1007/s00216-021-03343-2. Epub 2021 May 1.
Acid phosphatase has become a significant indicator of prognostic and medical diagnosis, and its dysfunction may lead to a series of diseases. A novel dual-signal fluorescence method for acid phosphatase detection based on europium polymer (europium-pyridine dicarboxylicacid-adenine) and pyridoxal phosphate (PLP) was proposed. PLP coordinated with europium polymer via Eu and P-O bonds, and the fluorescence of europium polymer was quenched due to the photoinduced electron transfer (PET) effect between aldehyde and europium polymer. Upon addition of acid phosphatase, the PLP was transformed to phosphate (Pi) and pyridoxal (PL). The PL was released from the surface of europium polymer, and the blue emission was enhanced due to the formation of internal hemiacetal, while the fluorescence of europium polymer recovered. The blue (PL) and red emission (Eu) were positively correlated with acid phosphatase activity; thus the sensitive assay of acid phosphatase was effectively achieved. The two signals were applied to determine the acid phosphatase with limits of detection (LOD) of 0.04 mU/mL and 0.38 mU/mL, and the linear ranges were 0.13-5.00 mU/mL and 1.25-20.00 mU/mL, respectively. The probe can be used to trace the acid phosphatase in biological systems and holds promise for use in clinical diagnosis and early prevention.
酸性磷酸酶已成为预后和医学诊断的重要指标，其功能障碍可能导致一系列疾病。本研究提出了一种基于铕聚合物（Eu-pyridine dicarboxylicacid-adenine）和磷酸吡哆醛（PLP）的新型双信号荧光法检测酸性磷酸酶。PLP 通过 Eu 和 P-O 键与铕聚合物配位，由于醛和铕聚合物之间的光诱导电子转移（PET）效应，铕聚合物的荧光被猝灭。加入酸性磷酸酶后，PLP 转化为磷酸盐（Pi）和吡哆醛（PL）。PL 从铕聚合物表面释放出来，由于形成内部半缩醛，蓝色发射增强，而铕聚合物的荧光恢复。蓝色（PL）和红色发射（Eu）与酸性磷酸酶活性呈正相关；因此，有效地实现了酸性磷酸酶的灵敏检测。这两个信号被应用于测定酸性磷酸酶，其检测限（LOD）分别为 0.04 mU/mL 和 0.38 mU/mL，线性范围分别为 0.13-5.00 mU/mL 和 1.25-20.00 mU/mL。该探针可用于追踪生物体系中的酸性磷酸酶，有望用于临床诊断和早期预防。
