Novel insights of impacts of solid content on high solid anaerobic digestion of cow manure: Kinetics and microbial community dynamics.

High solid anaerobic digestion has become the mainstream technology for sustainable on-farm treatment of solid wastes but has not been optimized with respect to increasing solid content in cow manure (CM). In the present study, CM was batch digested at total solid (TS) of 5%, 10%, 15% and 20% and microbial communities were investigated. The process remained stable up to 15% TS. The biomethane production rate at TS of 10% and 15% was reported to be 352.2 mL g VS and 318.6 mL g VS, reaching up to 83% and 75% of TS 5% biomethane, respectively. Kinetics results disclosed that the biodegradable organics could be utilized at increasing solid content with decreasing hydrolysis rate. The abundances of hydrogenotrophic and methylotrophic methanogens increased significantly with increasing solid content. This study is of great importance for understanding and application of high solid anaerobic digestion of cow manure.