Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China.
Department of Aquaculture, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China.
Tissue Cell. 2021 Oct;72:101551. doi: 10.1016/j.tice.2021.101551. Epub 2021 Apr 21.
Interleukin-8 (IL-8, CXCL8), a pro-inflammatory chemokine secreted by a variety of cell types, plays a critical role in the development of various immune diseases. Interactions between IL-8 and its receptor CXC receptor 1/2 (CXCR1/2) are known to promote chemotaxis and phagocytosis in many immune responses. In this study, we report the molecular characteristics and pharmacological activity of CXCR1 (MsCXCR1) in largemouth bass (Micropterus salmoides) and evaluated the functional involvement of MsCXCR1 in individuals infected with the pathogen Nocardia seriolae. MsCXCR1 was cloned into the pEGFP-N1 plasmid and the subcellular localization of MsCXCR1 on the cell membrane was verified in MsCXCR1-EGFP-expressing HEK293 cells. Following observation of receptor internalization and intracellular signaling detection, we further determined the functional interaction of secreted interleukin-8 (LcIL-8, the ligand for CXCR1 in large yellow croaker) and MsCXCR1 was further determined, and the ERK phosphorylation signal activation mediated by MsCXCR1 was demonstrated. Quantitative real-time PCR assays were conducted to analyze the transcriptional distribution of MsCXCR1 in various tissues of healthy and diseased largemouth bass. These results illustrate the significant elevation of MsCXCR1 expression in the head kidney, spleen and liver of M. salmoides, suggesting that MsCXCR1 was involved in the immune response in N. seriolae-infected largemouth bass and potentially affects the digestive function of this species.
白细胞介素-8（IL-8，CXCL8）是一种由多种细胞类型分泌的促炎趋化因子，在各种免疫疾病的发展中起着关键作用。IL-8 与其受体 CXC 受体 1/2（CXCR1/2）之间的相互作用已知可促进许多免疫反应中的趋化作用和吞噬作用。在这项研究中，我们报告了大口黑鲈（Micropterus salmoides）中 CXCR1（MsCXCR1）的分子特征和药理学活性，并评估了 MsCXCR1 在感染病原体诺卡氏菌（Nocardia seriolae）的个体中的功能参与。将 MsCXCR1 克隆到 pEGFP-N1 质粒中，并在表达 MsCXCR1-EGFP 的 HEK293 细胞中验证了 MsCXCR1 在细胞膜上的亚细胞定位。观察到受体内化和细胞内信号检测后，我们进一步确定了分泌的白细胞介素-8（LcIL-8，大黄鱼中 CXCR1 的配体）与 MsCXCR1 的功能相互作用，并证明了 MsCXCR1 介导的 ERK 磷酸化信号激活。进行定量实时 PCR 分析以分析健康和患病大口黑鲈各种组织中 MsCXCR1 的转录分布。这些结果表明，MsCXCR1 在头肾、脾脏和肝脏中的表达显著升高，这表明 MsCXCR1 参与了感染诺卡氏菌的大口黑鲈的免疫反应，并可能影响该物种的消化功能。
