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硫酸镁对早产缺氧缺血性大鼠模型视网膜的抗细胞凋亡和神经保护作用。

Possible antiapoptotic and neuroprotective effects of magnesium sulphate on retina in a preterm hypoxic-ischemic rat model.

机构信息

Department of Ophthalmology, Haydarpaşa Numune Training and Research Hospital, University of Health Sciences, İstanbul, Turkey

Department of Neonatology, Göztepe Training and Research Hospital, İstanbul Medeniyet University, İstanbul, Turkey

出版信息

Turk J Med Sci. 2021 Aug 30;51(4):2198-2205. doi: 10.3906/sag-2012-119.

DOI:10.3906/sag-2012-119
PMID:33932970
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8572035/
Abstract

BACKGROUND/AIM: The effects of systemic magnesium sulfate (MgSO4) on retina in preterm hypoxic-ischemic (HI) rat model are not known. Our aim was to investigate the effects of MgSO4 on retinal ganglion cell (RGC) count, retinal ganglion cell (RGC) apoptotic index, retinal vascular endothelial growth factor receptor-2 (VEGFR-2), and glial fibrillary acidic protein (GFAP) expressions in preterm HI rat model.

MATERIALS AND METHODS

Fifteen, postnatal day (PND) 7 rat pups were divided into 3 groups: 1. Sham-operated group, 2. HI group, and 3. MgSO4-treated HI group. The second and third groups underwent ischemia followed by exposure to hypoxia for 2 h (Vannucci model). The first and second groups received intraperitoneal saline and the third group received intraperitoneal MgSO4. On PND 10, eyes of the pups were evaluated for RGC count, apoptotic index, VEGFR-2, and GFAP expressions.

RESULTS

In both HI and MgSO4-treated HI group, the mean total RGC counts were found to be significantly decreased. However, the mean total RGC count in the MgSO4-treated HI group was significantly higher than that of the HI group. The mean apoptotic index was found to be significantly increased in the HI group. Retinal VEGFR-2 and GFAP expressions were found to be significantly higher in the HI group.

CONCLUSIONS

Magnesium sulfate preconditioning and treatment in preterm HI rat model might diminish apoptosis, relatively preserve RGCs, and reduce retinal VEGFR-2 and GFAP expressions.

摘要

背景/目的：目前尚不清楚全身硫酸镁（MgSO4）对早产儿缺氧缺血（HI）大鼠模型视网膜的影响。我们的目的是研究 MgSO4 对早产儿 HI 大鼠模型视网膜神经节细胞（RGC）计数、视网膜神经节细胞（RGC）凋亡指数、视网膜血管内皮生长因子受体-2（VEGFR-2）和胶质纤维酸性蛋白（GFAP）表达的影响。

材料和方法

15 只出生后第 7 天（PND7）的大鼠幼崽被分为 3 组：1. 假手术组，2. HI 组，和 3. MgSO4 处理的 HI 组。第二和第三组经历缺血，然后缺氧 2 小时（Vannucci 模型）。第一组和第二组接受腹腔内生理盐水，第三组接受腹腔内 MgSO4。在 PND10 时，评估幼崽的眼睛以进行 RGC 计数、凋亡指数、VEGFR-2 和 GFAP 表达。

结果

在 HI 组和 MgSO4 处理的 HI 组中，平均总 RGC 计数均明显减少。然而，MgSO4 处理的 HI 组的平均总 RGC 计数明显高于 HI 组。HI 组的平均凋亡指数明显增加。HI 组视网膜 VEGFR-2 和 GFAP 表达明显升高。

结论

在早产儿 HI 大鼠模型中，镁预处理和治疗可能减少凋亡，相对保留 RGC，并减少视网膜 VEGFR-2 和 GFAP 表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8fb/8572035/4ca7a19020f5/turkjmedsci-51-2198-fig002b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8fb/8572035/0dc1b8f330bc/turkjmedsci-51-2198-fig001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8fb/8572035/2cf713d4e434/turkjmedsci-51-2198-fig002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8fb/8572035/4ca7a19020f5/turkjmedsci-51-2198-fig002b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8fb/8572035/0dc1b8f330bc/turkjmedsci-51-2198-fig001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8fb/8572035/2cf713d4e434/turkjmedsci-51-2198-fig002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8fb/8572035/4ca7a19020f5/turkjmedsci-51-2198-fig002b.jpg

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