School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.
Chongqing Key Laboratory of Catalysis and New Environmental Materials, College of Environment and Resources, Chongqing Technology and Business University, Chongqing 400067, China.
Food Chem. 2021 Oct 1;358:129835. doi: 10.1016/j.foodchem.2021.129835. Epub 2021 Apr 20.
Simultaneous and ultra-sensitive detection strategy of Cu and Mg in wine and beer was developed based on dual DNA tweezers and entropy-driven three-dimensional DNA nanomachine. The dual DNAzyme can simultaneously respond to two kinds of metal ions and cause two kinds of "turn-on" fluorescent signals. The working principle of this strategy was indirectly proven. In addition, some key experimental parameters were also optimized. Under the optimum conditions, the limit of detection was 10 pM for Cu and 2 nM for Mg respectively which was significantly improved by entropy driven amplification. This strategy also showed good selectivity and specificity. It was successfully used to detect of Cu and Mg in wine and beer with 5.26% to 9.12% of relative standard deviation and 90.4% to 110.5% of recoveries.
基于双 DNA 镊子和熵驱动的三维 DNA 纳米机器，开发了一种用于同时超灵敏检测葡萄酒和啤酒中 Cu 和 Mg 的策略。双 DNA 酶可以同时响应两种金属离子并引发两种“开启”荧光信号。该策略的工作原理得到了间接证明。此外，还优化了一些关键的实验参数。在最佳条件下，Cu 的检测限为 10 pM，Mg 的检测限为 2 nM，这得益于熵驱动的扩增得到了显著提高。该策略还表现出良好的选择性和特异性。它成功地用于葡萄酒和啤酒中 Cu 和 Mg 的检测，相对标准偏差为 5.26%至 9.12%，回收率为 90.4%至 110.5%。
