Otorhinolaryngology-Department of Head and Neck Surgery, Faculty of Medicine, Canakkale Onsekiz Mart University, Turkey.
Health Services Vocational College, Çanakkale Onsekiz Mart University, 17100, Çanakkale, Turkey.
Mutat Res. 2021 Jan-Jun;822:111744. doi: 10.1016/j.mrfmmm.2021.111744. Epub 2021 Apr 22.
In the current study, we aimed to compare the level of genetic damages measured as micronucleus (MN), nucleoplasmic bridge (NPB), and nuclear bud formation (NBUD) in congenital hearing loss patients (n = 17) and control group (n = 24). The cytokinesis-blocked micronucleus assay (CBMN) was applied to the blood samples to measure the frequency of the markers in both groups. The frequencies of MN of hearing loss patients were found to be consistently significantly higher than those obtained for the control group (p < 0.0001). Similarly, we found significantly higher frequency of NPB in patients was obtained for the patient group (p < 0.0001). Finally, the frequencies of NBUD in patients is significantly higher than the level measured in the control group (p < 0.0001). Furthermore, the age-adjusted MNL, BNMN, NPB, and NBUD frequencies in the patients were significantly higher than those obtained in the control group. We observed that the frequency of MN in patients was positively correlated with NBUD frequency which may indicate a common mechanism for these biomarkers in the patient group. We found, for the first time, that there were statistically significant higher levels of MN, NPB, and NBUD in sensorineural hearing loss patients. Since the markers we evaluated were linked with crucial diseases, our findings might suggest that sensorineural hearing loss patients are susceptible to several crucial diseases, especially cancer. Furthermore, the results demonstrated the significance of the MN, NPB, and NBUD level and thus provides a potential marker for the diagnosis of congenital hearing loss patients.
在当前的研究中，我们旨在比较作为微核（MN）、核质桥（NPB）和核芽形成（NBUD）的遗传损伤水平，在先天性听力损失患者（n = 17）和对照组（n = 24）。我们应用胞质分裂阻断微核试验（CBMN）测量两组的标记物频率。听力损失患者的 MN 频率始终明显高于对照组（p < 0.0001）。同样，我们发现患者 NPB 的频率明显高于患者组（p < 0.0001）。最后，患者 NBUD 的频率明显高于对照组的水平（p < 0.0001）。此外，患者的年龄调整的 MNL、BNMN、NPB 和 NBUD 频率明显高于对照组。我们观察到患者的 MN 频率与 NBUD 频率呈正相关，这可能表明这些生物标志物在患者组中存在共同机制。我们首次发现，在感觉神经性听力损失患者中 MN、NPB 和 NBUD 的水平显著更高。由于我们评估的标志物与重要疾病有关，我们的发现可能表明感觉神经性听力损失患者易患几种重要疾病，特别是癌症。此外，结果表明 MN、NPB 和 NBUD 水平的重要性，因此为先天性听力损失患者的诊断提供了一个潜在的标志物。
