Ogunlade Babatunde, Fidelis Obinna P, Adelakun Sunday Aderemi
Human Anatomy Department, Federal University of Technology Akure, Akure, Nigeria.
Biomedical Technology Department, Federal University of Technology Akure, Akure, Nigeria.
Asian Spine J. 2022 Feb;16(1):92-98. doi: 10.31616/asj.2020.0479. Epub 2021 May 4.
Symptomatic disk degeneration is characterized by early pathological changes in the morphological, biochemical, and biomechanical properties of the intervertebral disc (IVD). Several methods that can be used to resolve this condition are being researched.
The present study was designed to examine the preventive and restorative properties of broccoli sprouts extracts in an annular puncture rabbit model of IVD degeneration.
IVD degeneration is closely associated with low back pain. A degenerated IVD is cytoarchitecturally characterized by a reduction in cell number, morphological changes in the disk tissue, and changes in the extracellular matrix contents. Methods, such as imaging and biochemical characterization, have been used for distinguishing between degenerated disk and non-degenerated disk.
Twenty New Zealand white rabbits (weighing approximately 2.0-3.5 kg) were divided into four groups (n=5). Group 1 received 50 mg/kg of normal saline solution orally for 4 weeks; group 2 received 50 mg/kg of normal saline solution immediately after puncture for 4 weeks; group 3 received 300 mg/kg of aqueous extract of broccoli sprouts orally immediately after puncture for 4 weeks; and group 4 received 300 mg/kg of aqueous extract of broccoli sprouts after 4 weeks of puncture for another 4 weeks. After the experiment, the disk height index (DHI), histomorphometry, and gene expression level were measured and analyzed.
The photomicrograph showed that the histological features of the punctured-treated groups (groups 3 and 4) were more similar to those of the non-punctured group than those of the punctured non-treated group. There was a significant difference in the DHI and relative gene expression levels among the non-treated punctured groups compared with those of the non-punctured and punctured-treated groups, respectively.
This study shows that the aqueous extract of broccoli sprouts exerts preventive and restorative effects in a rabbit model with disk degeneration.
有症状的椎间盘退变的特征是椎间盘（IVD）在形态、生化和生物力学特性方面出现早期病理变化。目前正在研究几种可用于解决这种情况的方法。
本研究旨在检测西兰花芽提取物在兔椎间盘退变环形穿刺模型中的预防和修复特性。
椎间盘退变与腰痛密切相关。退变的椎间盘在细胞结构上的特征是细胞数量减少、椎间盘组织形态改变以及细胞外基质成分变化。成像和生化特征等方法已被用于区分退变椎间盘和非退变椎间盘。
将20只新西兰白兔（体重约2.0 - 3.5千克）分为四组（n = 5）。第1组口服50毫克/千克生理盐水溶液，持续4周；第2组在穿刺后立即口服50毫克/千克生理盐水溶液，持续4周；第3组在穿刺后立即口服300毫克/千克西兰花芽水提取物，持续4周；第4组在穿刺4周后口服300毫克/千克西兰花芽水提取物，再持续4周。实验结束后，测量并分析椎间盘高度指数（DHI）、组织形态计量学和基因表达水平。
显微照片显示，穿刺治疗组（第3组和第4组）的组织学特征与未穿刺组比未穿刺未治疗组更相似。与未穿刺组和穿刺治疗组相比，未治疗穿刺组的DHI和相对基因表达水平分别存在显著差异。
本研究表明，西兰花芽水提取物在兔椎间盘退变模型中具有预防和修复作用。
