Fjösne H E, Strand H, Ostensen M A, Sunde A
Institute of Cancer Research, University Hospital, Trondheim, Norway.
Prostate. 1988;12(4):309-20. doi: 10.1002/pros.2990120404.
We have studied the activities of ornithine decarboxylase and adenosylmethionine decarboxylase in the 10(6)-m/s2 supernatants of the different lobes of the prostate and the seminal vesicles of castrates, androgen-stimulated castrates, and intact controls. After castration L-ornithine decarboxylase (ODC) and S-adenosyl-L-methionine decarboxylase (AMDC) activities fell in all tissues examined. The induction of kinetics was followed for 72 h after administration of testosterone propionate to castrated rats. AMDC activities increased more rapidly than ODC activities in every tissue studied. Peak activities were reached more rapidly in the dorsal lobe than in the other tissues. ODC activity of the ventral lobe increased linearly for 48 h after stimulation. In the other tissues studied, ODC activity reached a maximum after 24 h and thereafter leveled off or decreased. In conclusion we have found distinct differences in ODC and AMDC activity in various tissues and major differences between treatment groups, with near extinction of activity at castration. In castrates stimulated with testosterone, the between-group differences prevailed but with different patterns of ODC versus AMDC activity. AMDC is seemingly rate-limiting in polyamine synthesis in stimulated tissues, while ODC controls synthesis in tissues from castrated rats.
我们研究了去势大鼠、雄激素刺激的去势大鼠以及完整对照大鼠前列腺和精囊不同叶的10(6)-m/s2上清液中鸟氨酸脱羧酶和腺苷甲硫氨酸脱羧酶的活性。去势后，所有检测组织中的L-鸟氨酸脱羧酶（ODC）和S-腺苷-L-甲硫氨酸脱羧酶（AMDC）活性均下降。给去势大鼠注射丙酸睾酮后，对酶活性诱导动力学进行了72小时的跟踪研究。在每个研究组织中，AMDC活性比ODC活性增加得更快。背叶比其他组织更快达到峰值活性。刺激后，腹叶的ODC活性在48小时内呈线性增加。在其他研究组织中，ODC活性在24小时后达到最大值，此后趋于平稳或下降。总之，我们发现不同组织中ODC和AMDC活性存在明显差异，治疗组之间也存在主要差异，去势后活性几乎消失。在用睾酮刺激的去势大鼠中，组间差异仍然存在，但ODC与AMDC活性模式不同。在受刺激的组织中，AMDC似乎是多胺合成的限速酶，而ODC则控制去势大鼠组织中的合成。
