Käpyaho K, Pösö H, Jänne J
Biochem J. 1980 Oct 15;192(1):59-63. doi: 10.1042/bj1920059.
The effect of various hormones on the activities of the four enzymes engaged with the biosynthesis of the polyamines has been investigated in the rat. Human choriogonadotropin induced a dramatic, yet transient, stimulation of l-ornithine decarboxylase (EC 4.1.1.17) activity in rat ovary, with no or only marginal changes in the activities of S-adenosyl-l-methionine decarboxylase (EC 4.1.1.50), spermidine synthase (aminopropyltransferase; EC 2.5.1.16) or spermine synthase. A single injection of oestradiol into immature rats maximally induced uterine ornithine decarboxylase at 4h after the injection. This early stimulation of ornithine decarboxylase activity was accompanied by a distinct enhancement of adenosylmethionine decarboxylase activity and a decrease in the activities of spermidine synthase and spermine synthase. In the seminal vesicle of castrated rats, testosterone treatment elicited a striking and persistent stimulation of ornithine decarboxylase and adenosylmethionine decarboxylase activities. The activity of spermidine synthase likewise rapidly increased between the first and the second day after the commencement of the hormone treatment, whereas the activity of spermine synthase remained virtually unchanged during the whole period of observation. Testosterone-induced changes in polyamine formation in the ventral prostate were comparable with those found in the seminal vesicle, with the possible exception of a more pronounced stimulation of spermidine synthase activity. It thus appears that an enhancement in one or both of the propylamine transferase (aminopropyltransferase) activities in response to hormone administration is an indicator of hormone-dependent growth (uterus and the male accessory sexual glands), and is not necessarily associated with non-proliferative hormonal responses, such as gonadotropin-induced luteinization of the ovarian tissue.
已在大鼠中研究了各种激素对参与多胺生物合成的四种酶活性的影响。人绒毛膜促性腺激素可显著但短暂地刺激大鼠卵巢中L-鸟氨酸脱羧酶（EC 4.1.1.17）的活性，而S-腺苷-L-甲硫氨酸脱羧酶（EC 4.1.1.50）、亚精胺合酶（氨基丙基转移酶；EC 2.5.1.16）或精胺合酶的活性没有变化或仅有轻微变化。向未成熟大鼠单次注射雌二醇后，在注射后4小时可最大程度地诱导子宫鸟氨酸脱羧酶活性。鸟氨酸脱羧酶活性的这种早期刺激伴随着腺苷甲硫氨酸脱羧酶活性的明显增强以及亚精胺合酶和精胺合酶活性的降低。在去势大鼠的精囊中，睾酮处理可显著且持续地刺激鸟氨酸脱羧酶和腺苷甲硫氨酸脱羧酶的活性。在激素处理开始后的第一天到第二天之间，亚精胺合酶的活性同样迅速增加，而在整个观察期间精胺合酶的活性几乎保持不变。睾酮诱导的腹侧前列腺中多胺形成的变化与精囊中发现的变化相当，可能除了对亚精胺合酶活性的刺激更为明显之外。因此，似乎响应激素给药时丙胺转移酶（氨基丙基转移酶）一种或两种活性的增强是激素依赖性生长（子宫和雄性附属性腺）的指标，并且不一定与非增殖性激素反应相关，例如促性腺激素诱导的卵巢组织黄体化。
