Røkke O, Revhaug A, Osterud B, Giercksky K E
Department of Surgery, University Hospital of Tromsø, Norway.
Prog Clin Biol Res. 1988;272:247-62.
Changes in endotoxin levels and cardiovascular performance during antibiotic therapy in septicemia were investigated in a porcine model. One group of animals (n = 9) received gentamicin 2 mg/kg intravenously infusion two hours after induction of sepsis with live E. coli bacteria. Another group (n = 7) served as non-treated septic controls. Plasma-levels of endotoxin increased significantly after antibiotic administration from 0.26 +/- 0.02 ng/ml before treatment (0 hrs), to 1.1 +/- 0.3 ng/ml after two hours (p less than 0.01) and 2.1 +/- 0.98 ng/ml four hours after treatment (p less than 0.01). In the control group no significant increase occurred in the observation period. No difference could be demonstrated between the groups with regard to the number of live bacteria in blood, either before or after treatment. When the data from all the animals were taken together for the first two hours following antibiotic administration a significant negative correlation (p less than 0.05) was demonstrated between changes in endotoxin levels and cardiac output. This correlation was significant for animals in which the levels of endotoxin increased above 0.5 ng/ml (p less than 0.05). The present study indicates that endotoxin is liberated after antibiotic administration during bacteremia, and that this increase correlates with cardiac performance.
在猪模型中研究了败血症抗生素治疗期间内毒素水平和心血管功能的变化。一组动物（n = 9）在用活的大肠杆菌诱导败血症两小时后，静脉输注庆大霉素2 mg/kg。另一组（n = 7）作为未治疗的败血症对照。抗生素给药后，血浆内毒素水平显著升高，从治疗前（0小时）的0.26±0.02 ng/ml，升至两小时后的1.1±0.3 ng/ml（p<0.01），以及治疗四小时后的2.1±0.98 ng/ml（p<0.01）。在对照组中，观察期内未出现显著升高。两组在治疗前后血液中活菌数量方面均未显示出差异。当将抗生素给药后前两小时所有动物的数据汇总在一起时，内毒素水平变化与心输出量之间显示出显著的负相关（p<0.05）。对于内毒素水平升高至0.5 ng/ml以上的动物，这种相关性显著（p<0.05）。本研究表明，菌血症期间抗生素给药后内毒素被释放，且这种增加与心脏功能相关。
