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环状RNA hsa_circ_0013958通过调控miR-532-3p/WEE1轴在肝细胞癌中发挥致癌基因的作用。

Circular RNA hsa_circ_0013958 Functions as an Oncogenic Gene Through Modulating miR-532-3p/WEE1 Axis in Hepatocellular Carcinoma.

作者信息

Ma Tao, Ma Yue, Du Yongjun, Wei Zhongheng, Wang Jianchu, Jun Yufu, Xiao Fenqiang

机构信息

Department of Clinical Laboratory, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Department of Gastroenterology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

出版信息

Front Oncol. 2021 Apr 15;11:585172. doi: 10.3389/fonc.2021.585172. eCollection 2021.

DOI:10.3389/fonc.2021.585172
PMID:33937016
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8082454/
Abstract

BACKGROUND

circ0013958 was identified as a biomarker, which can be used for the diagnosis and screening of lung cancer. However, the role of circ0013958 in hepatocellular carcinoma (HCC) remains unclear.

METHODS

In our study, quantitative real-time polymerase chain reaction was performed to determine the levels of circ0013958 in HCC tissues and cell lines. EdU, CCK-8, transwell, flow cytometry and tumorigenesis assays were applied to assess the functions of circ0013958 in HCC and . Western blot assay was to detect the expression of WEE1. Luciferase reporter assay, bioinformatics analysis and rescue experiments were used to examine the interaction among circ0013958, miR-532-3p and WEE1.

RESULTS

It revealed that circ0013958 was significantly up-regulated in HCC, which was positively correlated with poor prognosis of HCC patients. Circ0013958 promoted HCC cell proliferation and invasion, inhibited cell apoptosis , and promoted tumorigenesis . Circ0013958 acted as a miR-532-3p sponge to regulate WEE1 expression, thus promoting the progression of HCC.

CONCLUSIONS

Circ0013958 promotes HCC progression through miR-532-3p/WEE1 axis. Circ0013958 may serve as a potential diagnostic biomarker and therapeutic target of HCC.

摘要

背景

circ0013958被鉴定为一种生物标志物，可用于肺癌的诊断和筛查。然而，circ0013958在肝细胞癌（HCC）中的作用仍不清楚。

方法

在我们的研究中，采用定量实时聚合酶链反应来测定HCC组织和细胞系中circ0013958的水平。应用EdU、CCK-8、Transwell、流式细胞术和肿瘤发生实验来评估circ0013958在HCC中的功能。蛋白质免疫印迹法检测WEE1的表达。采用荧光素酶报告基因实验、生物信息学分析和挽救实验来检测circ0013958、miR-532-3p和WEE1之间的相互作用。

结果

结果显示，circ0013958在HCC中显著上调，这与HCC患者的不良预后呈正相关。Circ0013958促进HCC细胞增殖和侵袭，抑制细胞凋亡，并促进肿瘤发生。Circ0013958作为miR-532-3p的海绵体来调节WEE1的表达，从而促进HCC的进展。

结论

Circ0013958通过miR-532-3p/WEE1轴促进HCC进展。Circ0013958可能作为HCC的潜在诊断生物标志物和治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a32e/8082454/ee82767c2ecc/fonc-11-585172-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a32e/8082454/a940d48fb739/fonc-11-585172-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a32e/8082454/78121a991092/fonc-11-585172-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a32e/8082454/33a5fa1e2a3d/fonc-11-585172-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a32e/8082454/175573078b45/fonc-11-585172-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a32e/8082454/cb05734049d5/fonc-11-585172-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a32e/8082454/ee82767c2ecc/fonc-11-585172-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a32e/8082454/a940d48fb739/fonc-11-585172-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a32e/8082454/78121a991092/fonc-11-585172-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a32e/8082454/33a5fa1e2a3d/fonc-11-585172-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a32e/8082454/175573078b45/fonc-11-585172-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a32e/8082454/cb05734049d5/fonc-11-585172-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a32e/8082454/ee82767c2ecc/fonc-11-585172-g006.jpg

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