衰老对小鼠和大鼠离体回肠胆碱能受体反应性影响的比较研究。

A comparative study of the effects of aging on the responsiveness of the cholinergic receptor of the isolated ileum of mouse and rat.

作者信息

Ochillo R F, Tsai C S

机构信息

Laboratory of Pharmacology, Xavier University of Louisiana, New Orleans 70125.

出版信息

Res Commun Chem Pathol Pharmacol. 1988 May;60(2):261-4.

PMID:3393734

Abstract

The responsiveness of the cholinergic receptor (ChR) of the isolated ileum of mouse and rat was characterized by determining the ED50 of acetylcholine (ACh) and pA2 of mepenzolate (MPZ) in tissues from animals of different ages. The ages (in months) of mice were 3, 6 and 18 and; 3, 6, 12 and 24 for rats respectively. The responsiveness of ChR in mice and rats decreased with age. There was no difference in the pA2 between the preparations derived from mice and rats nor was it affected by age. The results of this investigation suggest that (1) changes in the peripheral ChR with age may have potential in the understanding of central aging process and (2) the use of age rather than the weight of animal may provide reproducible data in studies using isolated preparations.

摘要

通过测定不同年龄动物组织中乙酰胆碱（ACh）的半数有效剂量（ED50）和甲哌卡因（MPZ）的拮抗参数（pA2），对小鼠和大鼠离体回肠的胆碱能受体（ChR）反应性进行了表征。小鼠的年龄（以月计）分别为3、6和18个月；大鼠的年龄分别为3、6、12和24个月。小鼠和大鼠ChR的反应性随年龄增长而降低。源自小鼠和大鼠的制剂之间的pA2没有差异，且不受年龄影响。本研究结果表明：（1）外周ChR随年龄的变化可能有助于理解中枢衰老过程；（2）在使用离体标本的研究中，使用年龄而非动物体重可能会提供可重复的数据。