Gallegos Maria, Hermes Alex, Patra Debjani
California State University, East Bay.
MicroPubl Biol. 2021 Apr 28;2021. doi: 10.17912/micropub.biology.000389.
is the only ortholog of mammalian PICALM/AP180, paralogs that play an important role in Clathrin-Mediated Endocytosis (CME) and the recycling of a subset of SNAREs, the vesicle-associated membrane proteins (VAMPs). In this publication we report the creation of a new allele that is endogenously-tagged with GFP just upstream of the stop codon. Moreover, we demonstrate that the UNC-11::GFP fusion protein functions like wild type with an expression pattern similar to UNC-11 antibody staining described previously.
它是哺乳动物PICALM/AP180的唯一直系同源物，这些旁系同源物在网格蛋白介导的内吞作用（CME）以及SNAREs（囊泡相关膜蛋白，VAMPs）的一个子集的循环利用中发挥重要作用。在本出版物中，我们报告了一个新等位基因的创建，该等位基因在终止密码子上游被内源性标记了绿色荧光蛋白（GFP）。此外，我们证明UNC-11::GFP融合蛋白的功能与野生型相似，其表达模式与先前描述的UNC-11抗体染色相似。
