Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, Changzhou University, Changzhou, China.
Changzhou University Huaide College, Jingjiang, China.
Chirality. 2021 Jul;33(7):385-396. doi: 10.1002/chir.23314. Epub 2021 May 2.
A composite chiral interface (BSA-MB-MWCNTs) was prepared from bovine serum albumin (BSA), methylene blue (MB), and multi-walled carbon nanotubes (MWCNTs) for chiral recognition of amine enantiomers (1S, 2S)-N,N'-dimethyl-1,2-cyclohexanediamine and (1R, 2R)-N,N'-dimethyl-1,2-cyclohexanediamine. The BSA-based composite was characterized by field emission scanning electron microscopy (FESEM) and ultraviolet-visible spectroscopy (UV-Vis). The electrochemical responses towards the two enantiomers were analyzed via cyclic voltammetry (CV), electrochemical AC impedance method (EIS), and differential-pulse voltammetry (DPV). The experimental results showed that the combination of MWCNTs and BSA could effectively improve the overall identification efficiency, and the peak current displayed by the S-enantiomer is larger, indicating that the prepared chiral surface has stronger interaction with the R-enantiomer. Under optimized condition, the current value of the oxidation peak of the chiral modified electrode showed a good linear relationship towards the amine concentration in the range of 5.0 × 10 to 5.0 × 10 mmol·L . The proposed electrochemical chiral interface is easy to handle and provides a promising electrochemical sensing platform that can be used to identify chiral amine enantiomers.
一种复合手性界面（BSA-MB-MWCNTs）由牛血清白蛋白（BSA）、亚甲蓝（MB）和多壁碳纳米管（MWCNTs）制备，用于手性识别胺对映体（1S，2S）-N，N'-二甲基-1,2-环己二胺和（1R，2R）-N，N'-二甲基-1,2-环己二胺。基于 BSA 的复合材料通过场发射扫描电子显微镜（FESEM）和紫外-可见光谱（UV-Vis）进行了表征。通过循环伏安法（CV）、电化学交流阻抗法（EIS）和差分脉冲伏安法（DPV）分析了两种对映体的电化学响应。实验结果表明，MWCNTs 和 BSA 的结合可以有效提高整体识别效率，并且 S-对映体显示的峰电流更大，表明所制备的手性表面与 R-对映体具有更强的相互作用。在优化条件下，手性修饰电极的氧化峰电流值与胺浓度在 5.0×10 至 5.0×10 之间呈现出良好的线性关系。所提出的电化学手性界面易于处理，提供了一种有前途的电化学传感平台，可用于识别手性胺对映体。
