Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119334, Russia.
Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.
Biochemistry (Mosc). 2021 Apr;86(4):506-516. doi: 10.1134/S0006297921040118.
Rathayibacter sp. VKM Ac-2759 (family Microbacteriaceae, class Actinobacteria) contains two glycopolymers in the cell wall. The main chain of rhamnan, glycopolymer 1, is built from the repeating tetrasaccharide units carrying terminal arabinofuranose residues at the non-reducing end, →3)-α-[α-D-Araf-(1→2)]-D-Rhap-(1→2)-α-D-Rhap-(1→3)-α-D-Rhap-(1→2)-α-D-Rhap-(1→. Similar to other described Rathayibacter species, rhamnose in the neutral glycopolymer of the VKM Ac-2759 strain is present in the D-configuration. Acetalated with pyruvic acid teichuronic acid, glycopolymer 2, is composed of the repeating tetrasaccharide units, →4)-β-D-GlcpA-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→3)-β-[4,6-S-Pyr]-D-Manp-(1→. Glycopolymers 1 and 2 were identified in prokaryotic microorganisms for the first time and their structures were established by chemical analysis and NMR spectroscopy. The obtained data can be used in taxonomic research, as well as for elucidating the mechanisms of plant colonization and infection by bacteria of the Rathayibacter genus.
节杆菌属 Rathayibacter sp. VKM Ac-2759（微杆菌科，放线菌纲）的细胞壁中含有两种糖聚合物。糖聚合物 1 是鼠李聚糖的主链，由带有末端阿拉伯呋喃糖残基的非还原端的重复四糖单元构建，→3））-α-[α-D-Araf-(1→2)]-D-Rhap-(1→2)-α-D-Rhap-(1→3)-α-D-Rhap-(1→2)-α-D-Rhap-(1→。与其他描述的节杆菌属物种类似，VKM Ac-2759 菌株中性糖聚合物中的鼠李糖呈 D-构型。经丙酮酸缩醛化的糖聚合物 2 由重复的四糖单元组成，→4））-β-D-GlcpA-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→3)-β-[4,6-S-Pyr]-D-Manp-(1→。糖聚合物 1 和 2 是首次在原核微生物中鉴定出来的，其结构通过化学分析和 NMR 光谱确定。所得数据可用于分类学研究，以及阐明细菌对植物定殖和感染的机制。
