在有或没有氨甲环酸存在的情况下，弹性蛋白酶对谷氨酸型和赖氨酸型纤溶酶原的降解作用。

Degradation of Glu- and Lys-plasminogen by elastase in the presence or absence of tranexamic acid.

作者信息

Takada A, Sugawara Y, Takada Y

机构信息

Department of Physiology, Hamamatsu University, School of Medicine, Shizuoka-ken, Japan.

出版信息

Thromb Res. 1988 Apr 15;50(2):285-94. doi: 10.1016/0049-3848(88)90229-0.

DOI:10.1016/0049-3848(88)90229-0

PMID:3394116

Abstract

Glu-plasminogen (Glu-plg) was degraded by elastase in the presence or absence of tranexamic acid. Glu-plg was degraded faster in the presence of tranexamic acid. Increase in the concentration of tranexamic acid resulted in increase in the appearance of degradation products, reaching a plateau level at 1 mM of tranexamic acid. Fifty percent increase in the concentration of one of degradation products was obtained at 0.22 mM of tranexamic acid, which is similar to a dissociation constant (Kd) of low affinity lysine binding sites (LBS) with tranexamic acid. As to the degradation rate of two isozymes of Glu-plg (Glu-plg I and II), Glu-plg II containing one carbohydrate chain was degraded faster than Glu-plg I containing two carbohydrate chains. Comparison of the degradation rates of Glu-plg and Lys-plg indicated that Lys-plg was degraded faster.

摘要

在有或没有氨甲环酸存在的情况下，谷氨酸纤溶酶原（Glu-plg）被弹性蛋白酶降解。在氨甲环酸存在时，Glu-plg降解得更快。氨甲环酸浓度的增加导致降解产物的出现增加，在1 mM氨甲环酸时达到平台水平。在0.22 mM氨甲环酸时，一种降解产物的浓度增加了50%，这与低亲和力赖氨酸结合位点（LBS）与氨甲环酸的解离常数（Kd）相似。至于Glu-plg的两种同工酶（Glu-plg I和II）的降解速率，含有一条碳水化合物链的Glu-plg II比含有两条碳水化合物链的Glu-plg I降解得更快。Glu-plg和赖氨酸纤溶酶原（Lys-plg）降解速率的比较表明，Lys-plg降解得更快。