Graduate Program in Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Department of Biophysics & Pharmacology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), Botucatu, São Paulo, Brazil.
Pharmacogenomics. 2021 Jun;22(8):451-464. doi: 10.2217/pgs-2021-0006. Epub 2021 May 4.
We examined the relationships between visfatin/NAMPT and nitrite concentrations (a marker of nitric oxide [NO] formation) or sFlt-1 levels in 205 patients with preeclampsia (PE) responsive or nonresponsive to antihypertensive therapy, and whether SNPs rs1319501 and rs3801266 affect nitrite concentrations in PE and 206 healthy pregnant women. Circulating visfatin/NAMPT and sFlt-1 levels were measured by ELISA, and nitrite concentrations by using an ozone-based chemiluminescence assay. In nonresponsive PE patients, visfatin/NAMPT levels were inversely related to nitrite concentrations and positively related to sFlt-1 levels. SNP rs1319501 affected nitrite concentrations in nonresponsive PE patients and was tightly linked with functional SNPs in Europeans. SNP rs1319501 and visfatin/NAMPT affect NO formation, sFlt-1 levels and antihypertensive therapy response in PE.
我们研究了 205 例先兆子痫（PE）患者对降压治疗有反应和无反应者中内脂素/NAMPT 与亚硝酸盐浓度（一氧化氮 [NO] 形成的标志物）或可溶性血管内皮生长因子受体 1（sFlt-1）水平之间的关系，以及 SNP rs1319501 和 rs3801266 是否影响 PE 中及 206 例健康孕妇的亚硝酸盐浓度。采用 ELISA 法测定循环内脂素/NAMPT 和 sFlt-1 水平，采用基于臭氧的化学发光法测定亚硝酸盐浓度。在无反应性 PE 患者中，内脂素/NAMPT 水平与亚硝酸盐浓度呈负相关，与 sFlt-1 水平呈正相关。SNP rs1319501 影响无反应性 PE 患者的亚硝酸盐浓度，与欧洲人群中的功能 SNP 紧密相关。SNP rs1319501 和内脂素/NAMPT 影响 PE 患者的 NO 形成、sFlt-1 水平和降压治疗反应。
