Department of Obstetrics and Gynecology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, China.
Department of Oncology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, China.
J Cell Mol Med. 2021 Jun;25(11):5335-5338. doi: 10.1111/jcmm.16488. Epub 2021 May 4.
The present study evaluated the anticancer potential of celastrol through down-regulation of matrix metalloproteinase-2 (MMP-2) and MMP-9. HeLa cells were incubated with different concentrations of celastrol (1, 10 and 100 µM) for 48h. Doxorubicin was used as a reference drug. Cancer cell migration, apoptosis, cell viability and mitochondrial fragmentation were evaluated following celastrol treatment. In addition, the expression level of MMP-2, MMP-9 and caspase-3 was evaluated following celastrol treatment. HeLa cell viability was 94.1 ± 7, 53.4 ± 4 and 36.3 ± 2% at 1-100 µM of celastrol, respectively. Apoptotic cell numbers were increased, and inhibition of larger wounds in cancer cells was observed following celastrol treatment. Celastrol-treated cells showed condensed nuclei and clumped mitochondria. Reduced expression of MMP-2 and MMP-9 and increased expression of caspase-3 were observed following celastrol treatment. Based on the experimental results, we are concluding that the celastrol was effective against HeLa cervical cancer cells.
本研究通过下调基质金属蛋白酶-2（MMP-2）和 MMP-9 来评估雷公藤红素的抗癌潜力。将 HeLa 细胞与不同浓度的雷公藤红素（1、10 和 100 μM）孵育 48 小时。阿霉素用作参考药物。用雷公藤红素处理后，评估癌细胞迁移、凋亡、细胞活力和线粒体碎片化。此外，还评估了雷公藤红素处理后 MMP-2、MMP-9 和 caspase-3 的表达水平。雷公藤红素在 1-100 μM 时，HeLa 细胞活力分别为 94.1±7%、53.4±4%和 36.3±2%。用雷公藤红素处理后，凋亡细胞数量增加，癌细胞中较大伤口的抑制作用观察到。雷公藤红素处理的细胞显示出浓缩的核和聚集的线粒体。用雷公藤红素处理后观察到 MMP-2 和 MMP-9 的表达减少，caspase-3 的表达增加。基于实验结果，我们得出结论，雷公藤红素对 HeLa 宫颈癌细胞有效。
