Tohamy Hossam G, El-Kazaz Sara E, Alotaibi Saqer S, Ibrahiem Hawary S, Shukry Mustafa, Dawood Mahmoud A O
Department of Pathology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Edfina 22758, Egypt.
Animals and Poultry Behavior and Management, Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Edfina 22758, Egypt.
Animals (Basel). 2021 Apr 30;11(5):1302. doi: 10.3390/ani11051302.
The study investigated the ability of boswellic acid (BA) to alleviate the testicular and oxidative injury FPN insecticide intoxication in the male rat model. Rats were randomly assigned to six equivalent groups (six rats each) as the following: control rats orally administered with 2 mL physiological saline/kg of body weight (bwt); boswellic acid (BA1) rats orally administered 250 mg BA/kg bwt; boswellic acid (BA2) rats orally administered 500 mg BA/kg bwt; fipronil (FPN) rats orally administered 20 mg FPN/kg bwt; (FPN + BA1) rats orally administered 20 mg FPN/kg bwt plus 250 mg BA/kg bwt, and (FPN + BA2) rats orally administered 20 mg FPN/kg bwt plus 500 mg BA/kg bwt. After 60 days, semen viability percentage and live spermatozoa percentage were decreased, and a considerably increased abnormality of the sperm cells in FPN-administered rats improved substantially with the co-administration of BA. BA had refinement of the histological architecture of testes and sexual glands. Quantitative analysis recorded a noticeable decline in the nuclear cell-proliferating antigen (PCNA) percentage area. FPN triggered cell damage, which was suggested by elevated malondialdehyde and interleukin 6, tumor necrosis factors alpha, and decreased glutathione level. Proapoptotic factor overexpression is mediated by FPN administration, while it decreased the antiapoptotic protein expression. Similarly, BA has shown significant upregulation in steroidogenic and fertility-related gene expression concerning the FPN group. Pathophysiological damages induced by FPN could be alleviated by BA's antioxidant ability and antiapoptotic factor alongside the upregulation of steroidogenic and fertility-related genes and regimented the detrimental effects of FPN on antioxidant and pro-inflammatory biomarkers.
该研究调查了乳香酸（BA）在雄性大鼠模型中减轻氟虫腈杀虫剂中毒所致睾丸和氧化损伤的能力。大鼠被随机分为六个等量组（每组六只），如下所示：对照组大鼠口服2 mL/千克体重的生理盐水；乳香酸（BA1）组大鼠口服250毫克/千克体重的BA；乳香酸（BA2）组大鼠口服500毫克/千克体重的BA；氟虫腈（FPN）组大鼠口服20毫克/千克体重的FPN；（FPN + BA1）组大鼠口服20毫克/千克体重的FPN加250毫克/千克体重的BA，以及（FPN + BA2）组大鼠口服20毫克/千克体重的FPN加500毫克/千克体重的BA。60天后，FPN处理组大鼠的精液活力百分比和活精子百分比降低，精子细胞异常显著增加，而联合给予BA后得到显著改善。BA使睾丸和性腺的组织学结构得到改善。定量分析记录到核细胞增殖抗原（PCNA）百分比面积显著下降。FPN引发细胞损伤，表现为丙二醛、白细胞介素6、肿瘤坏死因子α升高，谷胱甘肽水平降低。FPN给药介导促凋亡因子过表达，同时降低抗凋亡蛋白表达。同样，与FPN组相比，BA在类固醇生成和生育相关基因表达方面显示出显著上调。FPN引起的病理生理损伤可通过BA的抗氧化能力和抗凋亡因子以及类固醇生成和生育相关基因的上调得到缓解，并减轻FPN对抗氧化和促炎生物标志物的有害影响。
