Jurkow Rita, Kalisz Andrzej, Húska Dalibor, Sękara Agnieszka, Dastborhan Soheila
Department of Horticulture, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Kraków, Poland.
Laboratory of Plant Metabolomics and Epigenetics, Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic.
Nanomaterials (Basel). 2021 Apr 29;11(5):1171. doi: 10.3390/nano11051171.
Nowadays, there is an increasing interest in nanoparticle (NP) technology used in household and industrial products. It could cause an accumulation and dispersion of NPs in the environment, with possible harmful effects on living organisms. Nanoparticles significantly affect plants and alter their physiology and biochemical pathways, and nanotechnology can be used to improve plant characteristics that are desirable by humans. Therefore, more extensive studies of NP interactions with plants are still needed. The aim of this report is to investigate the effect of TiO nanoparticles (TiO-NPs) on the enzymatic and non-enzymatic antioxidants, fresh and dry weights, and malondialdehyde contents in oakleaf lettuce seedlings. Plants were foliar treated with a 0.75% suspension of TiO-NPs, while control plants were sprayed with deionized water. Leaves were sampled 4, 7, 9, 11, and 13 days after the treatment. The effects of TiO-NPs were time-dependent, but the most spectacular changes were observed 4 days after the treatment. Exposure of the plants to TiO-NPs significantly increased the contents of glutathione at all sampling points, total phenolics at days 4 and 13, and L-ascorbic acid at 4, 7, and 11 days after the treatment. Elevated levels of ascorbate peroxidase and guaiacol peroxidase activities were recorded at days 4 and 13, respectively. Total antioxidant capacity increased initially in treated seedlings, when compared with the control, and then decreased. On day 7, higher fresh and dry weights, as well as malondialdehyde contents in TiO-NPs treated plants were observed, compared with the control. The study demonstrated that the activation of some antioxidant system components due to TiO-NPs treatment was connected with the induction of mild oxidative stress, with no external symptoms of NP toxicity in oakleaf lettuce.
如今，人们对家用和工业产品中使用的纳米颗粒（NP）技术越来越感兴趣。它可能导致纳米颗粒在环境中积累和扩散，对生物有机体产生潜在有害影响。纳米颗粒会显著影响植物并改变其生理和生化途径，并且纳米技术可用于改善人类所需的植物特性。因此，仍需要对纳米颗粒与植物的相互作用进行更广泛的研究。本报告的目的是研究二氧化钛纳米颗粒（TiO-NPs）对橡叶生菜幼苗中酶促和非酶促抗氧化剂、鲜重和干重以及丙二醛含量的影响。用0.75%的TiO-NPs悬浮液对植物进行叶面处理，而对照植物喷洒去离子水。在处理后第4、7、9、11和13天对叶片进行采样。TiO-NPs的影响具有时间依赖性，但在处理后4天观察到最显著的变化。植物暴露于TiO-NPs后，在所有采样点谷胱甘肽含量显著增加，在处理后第4天和第13天总酚含量增加，在第4、7和11天L-抗坏血酸含量增加。在第4天和第13天分别记录到抗坏血酸过氧化物酶和愈创木酚过氧化物酶活性升高。与对照相比，处理后的幼苗总抗氧化能力最初增加，然后下降。在第7天，观察到TiO-NPs处理的植物鲜重和干重以及丙二醛含量均高于对照。该研究表明，TiO-NPs处理导致的一些抗氧化系统成分的激活与轻度氧化应激的诱导有关，橡叶生菜没有NP毒性的外部症状。
