Pan Xiaofei, Li Bao, Zhang Gan, Gong Yuyong, Liu Rui, Chen Benxin, Li Yang
Department of Colorectal and Anal Surgery, the Affiliated Hospital of West Anhui Health Vocational College, Lu'an, China.
Department of Burns and Orthopedic Surgery, the Affiliated Hospital of West Anhui Health Vocational College, Lu'an, China.
J Int Med Res. 2021 May;49(5):3000605211008338. doi: 10.1177/03000605211008338.
To evaluate the expression of retinoid-related orphan receptor gamma (RORγ) and its potential role in the prognosis of colon cancer.
The Cancer Genome Atlas and GSE117606 were used to evaluate to RORγ levels in colon cancer, and real-time quantitative polymerase chain reaction was applied for validation. UALCAN and MEXPRESS were used to analyze the associations of RORγ expression with clinical parameters. The survival analysis was conducted in GEPIA.
RORγ expression was significantly lower in colon tumors than in adjacent normal mucosa tissues. RORγ expression was significantly associated with tumor stage, lymph node metastasis, and liver metastasis. The area under the curve for diagnosis was 0.71. Decreased RORγ expression was positively correlated with the incidence of lymphatic invasion, microsatellite instability, the presence of residual tumor, venous invasion, and copy number variation. Overall survival was longer in patients with higher RORγ expression, especially those with microsatellite instability-high features. Methylation analysis revealed that hypermethylation of the RORγ promoter was associated with the colon cancer stage.
RORγ downregulation could be a potential biomarker for colon cancer, especially for predicting prognosis. Decreased RORγ expression in colon tumor may be associated with promoter hypermethylation.
评估维甲酸相关孤儿受体γ（RORγ）的表达及其在结肠癌预后中的潜在作用。
利用癌症基因组图谱和GSE117606评估结肠癌中RORγ水平，并应用实时定量聚合酶链反应进行验证。使用UALCAN和MEXPRESS分析RORγ表达与临床参数的相关性。在GEPIA中进行生存分析。
结肠癌组织中RORγ表达显著低于相邻正常黏膜组织。RORγ表达与肿瘤分期、淋巴结转移和肝转移显著相关。诊断曲线下面积为0.71。RORγ表达降低与淋巴浸润、微卫星不稳定性、残留肿瘤、静脉浸润和拷贝数变异的发生率呈正相关。RORγ表达较高的患者总生存期较长，尤其是具有微卫星高度不稳定特征的患者。甲基化分析显示，RORγ启动子的高甲基化与结肠癌分期相关。
RORγ下调可能是结肠癌的潜在生物标志物，尤其是用于预测预后。结肠癌中RORγ表达降低可能与启动子高甲基化有关。
