School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an, P. R. China.
Biofouling. 2021 Mar;37(3):341-351. doi: 10.1080/08927014.2021.1919649. Epub 2021 May 5.
Sanguinarine, a plant-derived benzophenanthridine alkaloid, was studied in terms of its anti-biofilm effects against carbapenem-resistant (CRSM). Minimum inhibitory concentrations (MICs) and cell membrane integrity were measured to investigate the antimicrobial mechanism of sanguinarine. Additionally, the extent of biofilm formation by CRSM exposed to sanguinarine was measured by crystal violet staining and visualized field emission scanning electron microscopy and confocal laser scanning microscopy. Sanguinarine displayed moderate activity against CRSM, with a MIC of 32 μg ml. Moreover, cell membrane integrity was severely disrupted by sanguinarine at 64 μg ml, and biofilm formation was sharply inhibited at 32 μg ml. The minimum biofilm eradication concentration was 512 μg ml against mature CRSM biofilms. The overall results suggest that sanguinarine is a potential anti-biofilm agent that can be explored to treat CRSM infections.
血根碱，一种植物衍生的苯并菲啶生物碱，在研究其抗生物膜作用对抗碳青霉烯耐药 （CRSM）。 测定最小抑菌浓度 （MIC） 和细胞膜完整性，以研究血根碱的抗菌机制。 此外，通过结晶紫染色和可视化场发射扫描电子显微镜和共聚焦激光扫描显微镜测量暴露于血根碱的 CRSM 生物膜形成的程度。 血根碱对 CRSM 表现出中等活性，MIC 为 32 μg ml。 此外，细胞膜完整性在 64 μg ml 时严重受损，生物膜形成在 32 μg ml 时急剧抑制。 针对成熟的 CRSM 生物膜，最小的生物膜清除浓度为 512 μg ml。 总体结果表明，血根碱是一种有潜力的抗生物膜剂，可用于治疗 CRSM 感染。
