Grace V M Berlin, Saranya S, Wilson D David
Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore, India.
School of Arts and Sciences, Karunya Institute of Technology and Sciences, Coimbatore, India.
J Histotechnol. 2021 Sep;44(3):127-138. doi: 10.1080/01478885.2021.1896291. Epub 2021 May 5.
Lung cancer is the leading cancer according to the World Health Organization (WHO), resulting in highest death rate worldwide due to the high level of metastasis. Hence, the drugs that protect from metastasis either as an adjuvant or a primary therapeutic agent may help to reduce the death rate. In this study, All Trans Retinoic Acid (ATRA) was tested for its action against metastatic lodging of B16F10 melanoma cells in the lung and liver of the C57BL/6 mouse model. Serum, lung and liver were evaluated biochemically for the cancer associated changes. Metastatic cancer development was confirmed by tumor nodule formation and histopathological analysis. RAR-β protein expression was analyzed by immunohistochemistry and histopathology. ATRA treated mice showed a percentage of inhibition on metastatic tumor growth in lung and liver and a corresponding protection against pathological changes in these organs. Cholesterol and γ-Glutamyl Transferase (GGT) levels found in cancer induced mice were reduced in the ATRA treated group. As compared to the normal group, lung tissue from cell line induced cancer control group had less RAR-β protein expression while the ATRA treated group showed enhanced RAR-β protein expression. This indicates that the anti-metastasis effects of ATRA might have shown the induction of RAR-β expression and subsequent molecular signaling pathways to regulate the homeostasis of biochemical changes. This study demonstrated the capability of ATRA to prevent the establishment of metastasis by the melanoma cell line into the lung and liver of experimental mice.
根据世界卫生组织（WHO）的数据，肺癌是主要的癌症，由于其高转移率导致全球最高的死亡率。因此，作为辅助剂或主要治疗剂来预防转移的药物可能有助于降低死亡率。在本研究中，测试了全反式维甲酸（ATRA）对B16F10黑色素瘤细胞在C57BL/6小鼠模型的肺和肝中转移定植的作用。对血清、肺和肝进行生化评估，以检测与癌症相关的变化。通过肿瘤结节形成和组织病理学分析确认转移性癌症的发展。通过免疫组织化学和组织病理学分析RAR-β蛋白表达。经ATRA处理的小鼠在肺和肝中对转移性肿瘤生长的抑制率以及对这些器官病理变化的相应保护作用。在经ATRA处理的组中，癌症诱导小鼠体内发现的胆固醇和γ-谷氨酰转移酶（GGT）水平降低。与正常组相比，细胞系诱导的癌症对照组的肺组织中RAR-β蛋白表达较少，而经ATRA处理的组显示RAR-β蛋白表达增强。这表明ATRA的抗转移作用可能表现为诱导RAR-β表达以及随后的分子信号通路，以调节生化变化的稳态。本研究证明了ATRA预防黑色素瘤细胞系在实验小鼠的肺和肝中建立转移的能力。
