Madri J A, Pratt B M, Yannariello-Brown J
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510.
Am J Pathol. 1988 Jul;132(1):18-27.
Current hypotheses suggest that the extracellular matrix modulates cellular function in physiologic homeostasis and during injury and repair and that selected cellular functions are cell and nuclear size dependent. For elucidation of the role of extracellular matrix-driven cell size changes in the modulation of endothelial cell proliferation and sheet migration an in vitro model system was used that allows for the culture of bovine aortic endothelial cells (BAEC) on various purified extracellular matrix components. BAEC exhibited distinct patterns in rates of attachment, spreading, migration, and proliferation when cultured on the selected extracellular matrix components laminin, types I and III collagen, type IV collagen, and fibronectin. In addition, there was a correlation between cell and nuclear size (on the various matrices tested) and rates of cell attachment and spreading. In contrast, an inverse correlation was noted between cell and nuclear size (on the various matrices tested) and proliferation and sheet migration. These data demonstrate that endothelial cells respond to matrix components in specific but complex fashions, mediated, in part, by changes in cell and nuclear size.
目前的假说认为，细胞外基质在生理稳态以及损伤和修复过程中调节细胞功能，而且特定的细胞功能取决于细胞和细胞核的大小。为了阐明细胞外基质驱动的细胞大小变化在内皮细胞增殖和片状迁移调节中的作用，使用了一种体外模型系统，该系统允许在各种纯化的细胞外基质成分上培养牛主动脉内皮细胞（BAEC）。当在选定的细胞外基质成分层粘连蛋白、I型和III型胶原蛋白、IV型胶原蛋白以及纤连蛋白上培养时，BAEC在附着、铺展、迁移和增殖速率方面表现出不同的模式。此外，（在测试的各种基质上）细胞和细胞核大小与细胞附着和铺展速率之间存在相关性。相反，（在测试的各种基质上）细胞和细胞核大小与增殖和片状迁移之间存在负相关。这些数据表明，内皮细胞以特定但复杂的方式对基质成分作出反应，部分是由细胞和细胞核大小的变化介导的。
