Departamento de Fisiología , Escuela Nacional de Ciencias Biológicas , Instituto Politécnico Nacional , Wilfrido Massieu esq. Manuel Stampa s/n , Col. Nueva Industrial Vallejo , Alcaldía Gustavo A. Madero , México City , Mexico ; Departamento de Fisiología y Farmacología , Centro de Ciencias Básicas , Universidad Autónoma de Aguascalientes , Ciudad Universitaria , Aguascalientes, Ags. , Mexico.
Departamento de Fisiología , Escuela Nacional de Ciencias Biológicas , Instituto Politécnico Nacional , Wilfrido Massieu esq. Manuel Stampa s/n , Col. Nueva Industrial Vallejo , Alcaldía Gustavo A. Madero , México City , Mexico.
Acta Neurobiol Exp (Wars). 2021;81(1):34-42. doi: 10.21307/ane-2021-005.
The kappa-opioid system (KOP) is the key in drug abuse. Of all the compounds isolated from (), salvinorin-A (Sal-A) is predominant. Further, Sal-A is the only compound within which is reported to have psychoactive properties as a powerful kappa-opioid receptor (KOPr) agonist. Based on the key role of the KOP system in the consumption of drugs, extract (SDE) and Sal-A may modify the alcohol intake in Wistar rats. Assessing voluntary alcohol intake as a drug consummatory behavior, food intake as natural reward behavior and tonic immobility as indicative of anxiety-like behavior, the present study sought to identify the role of both SDE and Sal-A in the Wistar rat model. Forty-eight adult male rats were randomly divided into six groups: control, alcohol naive and vehicle, alcohol-naive and SDE, alcohol-naive and Sal-A, alcohol-consumption and vehicle, alcohol-consumption and SDE, and alcohol-consumption and Sal-A. Alcohol and food intake were assessed for two weeks. In the middle of these two weeks, vehicle, SDE (containing ~1 mg/kg of Sal-A) or Sal-A was injected intraperitoneally once a day for a week. Tonic immobility testing was performed once. The administration of SDE produced a significant increase in voluntary alcohol intake especially in rats with a history of forced alcohol consumption from a juvenile age, Sal-A elicited an increase in alcohol intake in animals with or without previous alcohol exposure, SDE and Sal-A prolonged the tonic immobility duration and decreased food intake. In conclusion, or Sal-A stimulated alcohol consumption in rats with a history of alcohol intake and independent of previous exposure respectively, also SDE or Sal-A elicited an anorexigenic effect, and increased tonic immobility as indicative of anxious-like behavior. The kappa-opioid system (KOP) is the key in drug abuse. Of all the compounds isolated from (), salvinorin-A (Sal-A) is predominant. Further, Sal-A is the only compound within which is reported to have psychoactive properties as a powerful kappa-opioid receptor (KOPr) agonist. Based on the key role of the KOP system in the consumption of drugs, extract (SDE) and Sal-A may modify the alcohol intake in Wistar rats. Assessing voluntary alcohol intake as a drug consummatory behavior, food intake as natural reward behavior and tonic immobility as indicative of anxiety-like behavior, the present study sought to identify the role of both SDE and Sal-A in the Wistar rat model. Forty-eight adult male rats were randomly divided into six groups: control, alcohol naive and vehicle, alcohol-naive and SDE, alcohol-naive and Sal-A, alcohol-consumption and vehicle, alcohol-consumption and SDE, and alcohol-consumption and Sal-A. Alcohol and food intake were assessed for two weeks. In the middle of these two weeks, vehicle, SDE (containing ~1 mg/kg of Sal-A) or Sal-A was injected intraperitoneally once a day for a week. Tonic immobility testing was performed once. The administration of SDE produced a significant increase in voluntary alcohol intake especially in rats with a history of forced alcohol consumption from a juvenile age, Sal-A elicited an increase in alcohol intake in animals with or without previous alcohol exposure, SDE and Sal-A prolonged the tonic immobility duration and decreased food intake. In conclusion, or Sal-A stimulated alcohol consumption in rats with a history of alcohol intake and independent of previous exposure respectively, also SDE or Sal-A elicited an anorexigenic effect, and increased tonic immobility as indicative of anxious-like behavior.
κ-阿片系统（KOP）是药物滥用的关键。在所分离的化合物中，紫堇醇灵-A（Sal-A）是主要成分。此外，Sal-A 是唯一一种被报道具有致幻特性的化合物，可作为一种强大的κ-阿片受体（KOPr）激动剂。基于 KOP 系统在药物消费中的关键作用，紫堇提取物（SDE）和 Sal-A 可能会改变 Wistar 大鼠的酒精摄入量。评估自愿性酒精摄入量作为药物消耗行为，食物摄入量作为自然奖励行为，强直性不动作为焦虑样行为的指示，本研究旨在确定 SDE 和 Sal-A 在 Wistar 大鼠模型中的作用。48 只成年雄性大鼠随机分为六组：对照组、酒精初治组和载体组、酒精初治组和 SDE 组、酒精初治组和 Sal-A 组、酒精消耗组和载体组、酒精消耗组和 SDE 组、酒精消耗组和 Sal-A 组。两周内评估酒精和食物摄入量。在这两周的中间，每天一次给 SDE（含有~1mg/kg 的 Sal-A）或 Sal-A 腹腔注射，持续一周。一次进行强直性不动测试。SDE 的给药显著增加了自愿性酒精摄入量，特别是在幼年时被迫饮酒的大鼠中，Sal-A 在有或没有先前酒精暴露的动物中增加了酒精摄入量，SDE 和 Sal-A 延长了强直性不动的持续时间并减少了食物摄入量。总之，SDE 或 Sal-A 分别刺激有饮酒史和无先前暴露史的大鼠饮酒，SDE 或 Sal-A 还表现出厌食作用，并增加强直性不动，表明焦虑样行为。
