Xie Li, Fang Qianjin, Wei Xin, Zhou Ling, Wang Sheng
Department of Anesthesiology, the First Affiliated Hospital of University of Science and Technology of China, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.
Department of Obstetrics and Gynecology, the Second People's Hospital of Hefei, Anhui Medical University Affiliated Hefei Hospital, Hefei, Anhui, China.
J Neurophysiol. 2021 Jun 1;125(6):2117-2124. doi: 10.1152/jn.00124.2021. Epub 2021 May 5.
Sevoflurane anesthesia is correlated with the generation of postoperative cognitive dysfunction. Insulin-like growth factor 1 (IGF-1) has important function in the nervous system development. Intravenously injected IGF-1 is reported to successfully pass the blood-brain barrier and perform neuroprotection effect in the brain. Memory and learning abilities were analyzed through Morris water maze task. Relative levels of protein were examined through Western blot and enzyme-linked immunosorbent assay (ELISA). Relative mRNA levels were shown through quantitative real-time polymerase chain reaction (qRT-PCR). IGF-1 expression in the plasma and hippocampus was downregulated in sevoflurane anesthesia-induced rats and rescued by intravenous IGF-1 injection. In aged rats, intravenous injection of IGF-1 alleviated sevoflurane-caused cognitive injuries and elevated TNF-α, IL-1β, and IL-6 levels in the plasma and hippocampus and rescued sevoflurane-depressed Akt phosphorylation. In conclusion, the administration of IGF-1 through intravenous injection alleviates sevoflurane anesthesia-mediated neuroinflammation and cognitive impairment in rats. The effects of IGF-1 in this process may depend on its function in regulating the PI3K/Akt signaling pathway. IGF-1 expression was downregulated by sevoflurane anesthesia in rats and could be rescued by intravenous IGF-1 injection, which alleviated sevoflurane-caused cognitive injuries and enhanced inflammatory responses in aged rats. Intravenous injection of IGF-1 rescued sevoflurane-depressed Akt phosphorylation in aged rats.
七氟醚麻醉与术后认知功能障碍的发生相关。胰岛素样生长因子1（IGF-1）在神经系统发育中具有重要作用。据报道，静脉注射IGF-1能够成功通过血脑屏障并在脑中发挥神经保护作用。通过莫里斯水迷宫任务分析记忆和学习能力。通过蛋白质印迹法和酶联免疫吸附测定（ELISA）检测蛋白质的相对水平。通过定量实时聚合酶链反应（qRT-PCR）显示相对mRNA水平。在七氟醚麻醉诱导的大鼠中，血浆和海马中的IGF-1表达下调，静脉注射IGF-1可使其恢复。在老年大鼠中，静脉注射IGF-1减轻了七氟醚引起的认知损伤，提高了血浆和海马中TNF-α、IL-1β和IL-6的水平，并挽救了七氟醚抑制的Akt磷酸化。总之，静脉注射IGF-1可减轻大鼠七氟醚麻醉介导的神经炎症和认知障碍。IGF-1在此过程中的作用可能取决于其在调节PI3K/Akt信号通路中的功能。七氟醚麻醉使大鼠IGF-1表达下调，静脉注射IGF-1可使其恢复，这减轻了七氟醚引起的认知损伤并增强了老年大鼠的炎症反应。静脉注射IGF-1挽救了老年大鼠中七氟醚抑制的Akt磷酸化。
