Future Industries Institute, University of South Australia, Adelaide, Australia.
Methods Mol Biol. 2021;2228:159-166. doi: 10.1007/978-1-0716-1024-4_12.
Quantitation using mass spectrometry (MS) is a routine approach for multiple analytes, including small molecules and peptides. Electrospray-based MS platforms are typically employed, as they provide highly reproducible outputs for batch processing of multiple samples. Quantitation using matrix-assisted laser desorption/ionization (MALDI) time-of-flight (ToF) mass spectrometry, while less commonly adopted, offers the ability to monitor analytes at significantly higher throughput and lower cost compared with ESI MS. Achieving accurate quantitation using this approach requires the development of appropriate sample preparation, spiking of appropriate internal standards, and acquisition to minimize spot-to-spot variability. Here we describe the preparation of samples for accurate quantitation using MALDI-ToF MS. The methodology presented shows the ability to quantitate perfluorooctanesulfonic acid (PFOS) from contaminated water.
使用质谱（MS）进行定量分析是一种常规方法，可用于多种分析物，包括小分子和肽。通常采用基于电喷雾的 MS 平台，因为它们为批量处理多个样品提供了高度可重复的输出。与 ESI-MS 相比，使用基质辅助激光解吸/电离（MALDI）飞行时间（ToF）质谱进行定量分析的应用虽然较少，但具有更高的分析物通量和更低的成本的优势。采用这种方法实现准确定量需要开发适当的样品制备方法、合适的内标物添加以及采集方法来最小化斑点间的变异性。在这里，我们描述了使用 MALDI-ToF-MS 进行准确定量分析的样品制备方法。所提出的方法表明能够定量检测受污染水中的全氟辛烷磺酸（PFOS）。
