鉴定与真核生物mRNA帽结构交联的80 kDa蛋白质为起始因子eIF-4B。
Identification of the 80-kDa protein that crosslinks to the cap structure of eukaryotic mRNAs as initiation factor eIF-4B.
作者信息
Milburn S C, Pelletier J, Sonenberg N, Hershey J W
机构信息
Department of Biological Chemistry, School of Medicine, University of California, Davis 95616.
出版信息
Arch Biochem Biophys. 1988 Jul;264(1):348-50. doi: 10.1016/0003-9861(88)90604-2.
PMID:3395129
Abstract
Preparations of either crude or purified protein synthesis initiation factors, when tested by crosslinking to the m7G-cap structure of mRNAs, exhibit specific crosslinking to an 80-kDa protein. Polyclonal antibodies specific for eIF-4B precipitate the 80-kDa cap-radiolabeled protein, thereby demonstrating that eIF-4B binds mRNA near its 5'-terminus.
摘要
当通过与mRNA的m7G帽结构交联进行测试时，粗制或纯化的蛋白质合成起始因子制剂均显示出与一种80 kDa蛋白质的特异性交联。针对eIF-4B的多克隆抗体沉淀出80 kDa帽放射性标记的蛋白质，从而证明eIF-4B在其5'末端附近结合mRNA。
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