对细胞核中转录的“精准”理解
Towards a 'Spot On' Understanding of Transcription in the Nucleus.
作者信息
Patange Simona, Ball David A, Karpova Tatiana S, Larson Daniel R
机构信息
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, United States.
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, United States.
出版信息
J Mol Biol. 2021 Jul 9;433(14):167016. doi: 10.1016/j.jmb.2021.167016. Epub 2021 May 2.
Abstract
Regulation of transcription by RNA Polymerase II (RNAPII) is a rapidly evolving area of research. Technological developments in microscopy have revealed insight into the dynamics, structure, and localization of transcription components within single cells. A frequent observation in many studies is the appearance of 'spots' in cell nuclei associated with the transcription process. In this review we highlight studies that characterize the temporal and spatial characteristics of these spots, examine possible pitfalls in interpreting these kind of imaging data, and outline directions where single-cell imaging may advance in ways to further our understanding of transcription regulation.
摘要
RNA聚合酶II（RNAPII）介导的转录调控是一个快速发展的研究领域。显微镜技术的发展使人们对单细胞内转录组件的动态变化、结构及定位有了更深入的了解。在许多研究中经常观察到，细胞核中会出现与转录过程相关的“斑点”。在这篇综述中，我们重点介绍了一些研究，这些研究描述了这些斑点的时空特征，探讨了解释这类成像数据时可能存在的陷阱，并概述了单细胞成像在推进我们对转录调控理解方面可能取得进展的方向。
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