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精子洗涤培养基在提高精子活力方面的功效。

Efficacy of sperm wash media in improving sperm motility.

作者信息

Daru J, Mathur S, Williamson H O

机构信息

Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston 29425.

出版信息

Arch Androl. 1988;20(2):113-5. doi: 10.3109/01485018808987060.

DOI:10.3109/01485018808987060
PMID:3395154
Abstract

Spermatozoa from 15 fertile men were washed with Ham's F10 and incubated with two commercially available sperm nutrient media for 2, 4, 6, and 24 h. Both sperm capacitation medium (Irvine Scientific Co., Santa Ana, CA) and Pro-ception (Milex Products, Inc., Chicago, IL) proved to be capable of improving sperm motion characteristics. These media may be used for incubating sperm for intrauterine insemination or for in vitro fertilization.

摘要

从15名生育能力正常的男性体内获取的精子,用哈姆F10培养液洗涤后,与两种市售精子营养培养基一起孵育2小时、4小时、6小时和24小时。结果证明,精子获能培养基(加利福尼亚州圣安娜市欧文科学公司)和Pro-ception(伊利诺伊州芝加哥市米莱克斯产品公司)均能改善精子运动特性。这些培养基可用于孵育精子以进行宫内人工授精或体外受精。

相似文献

1
Efficacy of sperm wash media in improving sperm motility.精子洗涤培养基在提高精子活力方面的功效。
Arch Androl. 1988;20(2):113-5. doi: 10.3109/01485018808987060.
2
Intrauterine insemination. Effect of wash media on pregnancy rates and sperm motility.宫内人工授精。冲洗介质对妊娠率和精子活力的影响。
J Reprod Med. 1993 Sep;38(9):711-5.
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Absence of glucose decreases human fertilization and sperm movement characteristics in vitro.体外缺乏葡萄糖会降低人类受精率和精子运动特性。
Hum Reprod. 1997 Jan;12(1):119-23. doi: 10.1093/humrep/12.1.119.
4
Effect of washing and capacitating media pH on bull sperm motility, acrosome integrity, and ability to penetrate zona-free hamster oocytes.洗涤和获能培养基pH值对公牛精子活力、顶体完整性及穿透去透明带仓鼠卵母细胞能力的影响。
J Dairy Sci. 1989 Oct;72(10):2691-9. doi: 10.3168/jds.S0022-0302(89)79411-X.
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Incubation of mouse sperm with lactate delays capacitation and hyperactivation and lowers fertilization levels in vitro.
Gamete Res. 1988 Aug;20(4):459-73. doi: 10.1002/mrd.1120200407.
6
Snow leopard (Panthera uncia) spermatozoa are sensitive to alkaline pH, but motility in vitro is not influenced by protein or energy supplements.雪豹(Panthera uncia)精子对碱性pH敏感,但体外活力不受蛋白质或能量补充剂的影响。
J Androl. 1996 Sep-Oct;17(5):558-66.
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Comparison of bicarbonate and HEPES-buffered media on pregnancy rates after intrauterine insemination with cryopreserved donor sperm.
Fertil Steril. 1991 Sep;56(3):540-6. doi: 10.1016/s0015-0282(16)54555-5.
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Chemical composition and protein source in the capacitation medium significantly affect the ability of human spermatozoa to undergo follicular fluid induced acrosome reaction.获能培养基中的化学成分和蛋白质来源显著影响人类精子发生卵泡液诱导的顶体反应的能力。
Hum Reprod. 1993 Apr;8(4):575-80. doi: 10.1093/oxfordjournals.humrep.a138099.
9
Pregnant women's serum provides a novel support for human sperm hyperactivated motility.孕妇血清为人类精子的超活化运动提供了一种新的支持。
Int J Fertil Menopausal Stud. 1994 Nov-Dec;39(6):324-32.
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Techniques of sperm selection, improvement and separation in an in vitro fertilization program.体外受精程序中精子选择、改善及分离技术
Acta Eur Fertil. 1989 Sep-Oct;20(5):269-83.

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